PMID- 23201004
OWN - NLM
STAT- MEDLINE
DCOM- 20130415
LR  - 20171116
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1270
DP  - 2012 Dec 28
TI  - Zwitterionic silica-based monolithic capillary columns for isocratic and gradient 
      hydrophilic interaction liquid chromatography.
PG  - 178-85
LID - S0021-9673(12)01687-1 [pii]
LID - 10.1016/j.chroma.2012.11.005 [doi]
AB  - This study introduces zwitterionic monolithic capillary columns intended for 
      isocratic and gradient HILIC separations. Silica-based monolithic capillary 
      columns (100 mum x 150 mm) prepared by acidic hydrolysis of tetramethoxysilane in 
      the presence of polyethylene glycol and urea were modified by a sulfoalkylbetaine 
      zwitterion ([2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium 
      hydroxide) to HILIC stationary phase. The prepared columns were evaluated under 
      the isocratic and gradient separation conditions using a standard mixture 
      containing nucleic acid bases, nucleosides, and 2-deoxynucleosides. Mobile phases 
      contained high concentration of acetonitrile (95-85%, v/v) and 5-50mM ammonium 
      acetate or ammonium formate in the pH range of 3-6. The synthesized columns 
      showed a long-term stability under the separation conditions while the high 
      permeability and efficiency originating from dual structure of the silica 
      monolith were preserved. The relative standard deviations (RSDs) for the 
      retention times of tested solutes were lower than 2% under the isocratic 
      conditions and lower than 3.5% under the gradient conditions.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Moravcova, Dana
AU  - Moravcova D
AD  - Institute of Analytical Chemistry of the ASCR, v. v. i., Veveri 97, 60200 Brno, 
      Czech Republic. moravcova@iach.cz
FAU - Planeta, Josef
AU  - Planeta J
FAU - Kahle, Vladislav
AU  - Kahle V
FAU - Roth, Michal
AU  - Roth M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121114
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Acetonitriles)
RN  - 0 (Nucleic Acids)
RN  - 0 (Nucleosides)
RN  - 3SCV180C9W (Betaine)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 8CVU22OCJW (sulfobetaine)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetonitriles/chemistry
MH  - Betaine/analogs & derivatives/chemistry
MH  - Chromatography, Liquid/*instrumentation/methods
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Nucleic Acids/chemistry/isolation & purification
MH  - Nucleosides/chemistry/isolation & purification
MH  - Reproducibility of Results
MH  - Silicon Dioxide/*chemistry
EDAT- 2012/12/04 06:00
MHDA- 2013/04/16 06:00
CRDT- 2012/12/04 06:00
PHST- 2012/07/31 00:00 [received]
PHST- 2012/10/31 00:00 [revised]
PHST- 2012/11/01 00:00 [accepted]
PHST- 2012/12/04 06:00 [entrez]
PHST- 2012/12/04 06:00 [pubmed]
PHST- 2013/04/16 06:00 [medline]
AID - S0021-9673(12)01687-1 [pii]
AID - 10.1016/j.chroma.2012.11.005 [doi]
PST - ppublish
SO  - J Chromatogr A. 2012 Dec 28;1270:178-85. doi: 10.1016/j.chroma.2012.11.005. Epub 
      2012 Nov 14.