PMID- 23201089
OWN - NLM
STAT- MEDLINE
DCOM- 20130703
LR  - 20130125
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 187
IP  - 2
DP  - 2013 Feb
TI  - Enhanced immunogenicity induced by an alphavirus replicon-based pseudotyped 
      baculovirus vaccine against porcine reproductive and respiratory syndrome virus.
PG  - 251-8
LID - S0166-0934(12)00398-9 [pii]
LID - 10.1016/j.jviromet.2012.11.018 [doi]
AB  - Pseudotyped baculovirus has emerged as a promising vector for vaccine development 
      and gene therapy. Alphaviruses, such as Semliki Forest virus (SFV), have also 
      received considerable attention for use as expression vectors because of their 
      self-replicating properties. In this study, pseudotyped baculovirus containing 
      the hybrid cytomegalovirus (CMV) promoter/SFV replicon was used as a vector to 
      co-express the GP5 and M proteins of porcine reproductive and respiratory 
      syndrome virus (PRRSV). The immunogenicity of the resulting recombinant 
      baculovirus (BV-SFV-5m6) was compared with the pseudotyped baculovirus vaccine 
      (BV-CMV-5m6), in which the expression of GP5 and M were driven by the CMV 
      promoter only. In vitro, BV-SFV-5m6 exhibited enhanced expression of foreign 
      proteins and also caused apoptosis in transduced cells. After immunization in 
      BALB/c mice, BV-SFV-5m6 induced strong GP5-specific ELISA antibodies and 
      neutralizing antibodies against homologous and heterologous viruses, along with 
      dose sparing. Further analysis of the cell-mediated immune response showed that 
      BV-SFV-5m6 elicited a Th1-dominant immune response that was greater than that 
      elicited by BV-CMV-5m6. Taken together, the results of this study indicate that a 
      baculovirus containing the hybrid CMV promoter/alphavirus replicon can be 
      utilized as an alternative strategy to develop an efficacious vaccine against 
      PRRSV infection.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Wu, Qunfeng
AU  - Wu Q
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, China.
FAU - Xu, Fengqin
AU  - Xu F
FAU - Fang, Liurong
AU  - Fang L
FAU - Xu, Jinfang
AU  - Xu J
FAU - Li, Bin
AU  - Li B
FAU - Jiang, Yunbo
AU  - Jiang Y
FAU - Chen, Huanchun
AU  - Chen H
FAU - Xiao, Shaobo
AU  - Xiao S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121128
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Drug Carriers)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Baculoviridae/genetics
MH  - Drug Carriers/*administration & dosage
MH  - Female
MH  - Genetic Vectors
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Semliki forest virus/genetics
MH  - Th1 Cells/immunology
MH  - Vaccines, Synthetic/administration & dosage/genetics/immunology
MH  - Viral Envelope Proteins/biosynthesis/genetics/immunology
MH  - Viral Matrix Proteins/biosynthesis/genetics/immunology
MH  - Viral Vaccines/administration & dosage/genetics/*immunology
EDAT- 2012/12/04 06:00
MHDA- 2013/07/05 06:00
CRDT- 2012/12/04 06:00
PHST- 2011/11/29 00:00 [received]
PHST- 2012/10/14 00:00 [revised]
PHST- 2012/11/13 00:00 [accepted]
PHST- 2012/12/04 06:00 [entrez]
PHST- 2012/12/04 06:00 [pubmed]
PHST- 2013/07/05 06:00 [medline]
AID - S0166-0934(12)00398-9 [pii]
AID - 10.1016/j.jviromet.2012.11.018 [doi]
PST - ppublish
SO  - J Virol Methods. 2013 Feb;187(2):251-8. doi: 10.1016/j.jviromet.2012.11.018. Epub 
      2012 Nov 28.