PMID- 23202722
OWN - NLM
STAT- MEDLINE
DCOM- 20130812
LR  - 20211021
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 73
IP  - 3
DP  - 2013 Mar
TI  - Ovine uterine space restriction alters placental transferrin receptor and fetal 
      iron status during late pregnancy.
PG  - 277-85
LID - 10.1038/pr.2012.180 [doi]
AB  - BACKGROUND: Fetal growth restriction is reported to be associated with impaired 
      placental iron transport. Transferrin receptor (TfR) is a major placental iron 
      transporter in humans but has not been studied in sheep. TfR is regulated by both 
      iron and nitric oxide (NO), the molecule produced by endothelial nitric oxide 
      synthase (eNOS). We hypothesized that limited placental development downregulates 
      both placental TfR and eNOS expression, thereby lowering fetal tissue iron. 
      METHODS: An ovine surgical uterine space restriction (USR) model, combined with 
      multifetal gestation, tested the extremes of uterine and placental adaptation. 
      Blood, tissues, and placentomes from non-space restricted (NSR) singletons were 
      compared with USR fetuses at gestational day (GD) 120 or 130. RESULTS: When 
      expressed proportionate to fetal weight, liver iron content did not differ, 
      whereas renal iron was higher in USR vs. NSR fetuses. Renal TfR protein 
      expression did not differ, but placental TfR expression was lower in USR fetuses 
      at GD130. Placental levels of TfR correlated to eNOS. TfR was localized 
      throughout the placentome, including the hemophagous zone, implicating a role for 
      TfR in ovine placental iron transport. CONCLUSION: Fetal iron was regulated in an 
      organ-specific manner. In USR fetuses, NO-mediated placental adaptations may 
      prevent the normal upregulation of placental TfR at GD130.
FAU - Sun, Mary Y
AU  - Sun MY
AD  - Department of Pediatrics, Perinatal Research Laboratory, University of Wisconsin, 
      Madison, Wisconsin, USA.
FAU - Habeck, Jason M
AU  - Habeck JM
FAU - Meyer, Katie M
AU  - Meyer KM
FAU - Koch, Jill M
AU  - Koch JM
FAU - Ramadoss, Jayanth
AU  - Ramadoss J
FAU - Blohowiak, Sharon E
AU  - Blohowiak SE
FAU - Magness, Ronald R
AU  - Magness RR
FAU - Kling, Pamela J
AU  - Kling PJ
LA  - eng
GR  - HL087144/HL/NHLBI NIH HHS/United States
GR  - HL49210/HL/NHLBI NIH HHS/United States
GR  - HD38843/HD/NICHD NIH HHS/United States
GR  - R01 HL087144/HL/NHLBI NIH HHS/United States
GR  - P01 HD038843/HD/NICHD NIH HHS/United States
GR  - R01 HL049210/HL/NHLBI NIH HHS/United States
GR  - R01 HL117341/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20121130
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Receptors, Transferrin)
RN  - E1UOL152H7 (Iron)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Blotting, Western
MH  - Body Weights and Measures
MH  - Female
MH  - Fetus
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Immunohistochemistry
MH  - Iron/*metabolism
MH  - Kidney/anatomy & histology/metabolism
MH  - Nitric Oxide Synthase Type III/*metabolism
MH  - Organ Size/physiology
MH  - Placenta/*metabolism
MH  - Placentation
MH  - Pregnancy
MH  - Receptors, Transferrin/*metabolism
MH  - Sheep
MH  - Uterus/anatomy & histology/*physiology
PMC - PMC3678369
MID - NIHMS447508
EDAT- 2012/12/04 06:00
MHDA- 2013/08/13 06:00
PMCR- 2013/09/01
CRDT- 2012/12/04 06:00
PHST- 2012/12/04 06:00 [entrez]
PHST- 2012/12/04 06:00 [pubmed]
PHST- 2013/08/13 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - pr2012180 [pii]
AID - 10.1038/pr.2012.180 [doi]
PST - ppublish
SO  - Pediatr Res. 2013 Mar;73(3):277-85. doi: 10.1038/pr.2012.180. Epub 2012 Nov 30.