PMID- 23203848
OWN - NLM
STAT- MEDLINE
DCOM- 20130418
LR  - 20211021
IS  - 1749-0774 (Electronic)
IS  - 0914-7470 (Linking)
VI  - 25
IP  - 4
DP  - 2012 Dec
TI  - Successful second delivery outcome using refrozen thawed testicular sperm from an 
      infertile male true hermaphrodite with a 46, XX/46, XY karyotype: case report.
PG  - 96-9
LID - 10.1007/s13577-012-0054-3 [doi]
AB  - We report a successful second delivery of a healthy infant fathered using 
      refrozen thawed testicular sperm from an infertile male chimera. We also examined 
      sex chromosome distribution of the seminiferous tubule. Intracytoplasmic sperm 
      injection (ICSI) was performed using the remaining refrozen testicular sperm, 
      which had been stored during the first treatment. Biopsied testicular cells were 
      examined by fluorescence in situ hybridization (FISH) and the peripheral 
      lymphocyte karyotype was tested using a G-band. Following ICSI, a second 
      pregnancy was established, and a healthy girl was successfully delivered at 40 
      gestational weeks without complications. Although the husband's lymphocyte 
      chromosomal analysis revealed a 46, XX [28]/46, XY [2] karyotype, the 
      seminiferous tubule cells on histological examination by FISH were chimeric sex 
      chromosome type XX [18]/XY [82]. In conclusion, this is a very rare case report 
      of a successful subsequent delivery of a healthy infant (46, XX) from an 
      infertile true hermaphrodite (46, XX/46, XY) using refrozen thawed testicular 
      sperm. The seminiferous tubule cells' karyotype ratio differed from that of the 
      lymphocytes.
FAU - Sugawara, Nobuo
AU  - Sugawara N
AD  - Iwaki Women's Clinic, 3-2 Ookishita, Uchigo, Iwaki, Fukushima 973-8403, Japan. 
      sugawara@iwaki-fu.or.jp
FAU - Kimura, Yasuyuki
AU  - Kimura Y
FAU - Araki, Yasuhisa
AU  - Araki Y
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20121201
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
SB  - IM
MH  - Azoospermia/genetics/*therapy
MH  - Chimera
MH  - *Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - *Cryopreservation
MH  - Female
MH  - Humans
MH  - Infant, Newborn
MH  - *Karyotype
MH  - Lymphocytes/cytology
MH  - Male
MH  - Ovotesticular Disorders of Sex Development/genetics/*therapy
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Seminiferous Tubules/cytology
MH  - Sperm Injections, Intracytoplasmic
MH  - *Spermatozoa
EDAT- 2012/12/04 06:00
MHDA- 2013/04/20 06:00
CRDT- 2012/12/04 06:00
PHST- 2012/08/22 00:00 [received]
PHST- 2012/10/10 00:00 [accepted]
PHST- 2012/12/04 06:00 [entrez]
PHST- 2012/12/04 06:00 [pubmed]
PHST- 2013/04/20 06:00 [medline]
AID - 10.1007/s13577-012-0054-3 [doi]
PST - ppublish
SO  - Hum Cell. 2012 Dec;25(4):96-9. doi: 10.1007/s13577-012-0054-3. Epub 2012 Dec 1.