PMID- 23207873
OWN - NLM
STAT- MEDLINE
DCOM- 20130805
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 36
IP  - 2
DP  - 2013
TI  - Suppressive effects of oral administration of heat-killed Lactobacillus 
      acidophilus on T helper-17 immune responses in a bovine 
      beta-lactoglobulin-sensitized mice model.
PG  - 202-7
AB  - The present study was planned to explore the effect of Lactobacillus (L.) 
      acidophilus on the T helper-17 (Th17) immune response in a mouse model of 
      beta-lactoglobulin (beta-lg) allergy. Bovine beta-lg sensitised BALB/c mice were orally 
      administered with different doses of heat-killed L. acidophilus (low, 5x10(7) 
      colony forming unit (CFU); medium, 5x10(8) CFU; high, 5x10(9) CFU) in 200 microL of 
      phosphate buffered saline (PBS) three times a week, starting from 1 week before 
      beta-lg sensitisation for 4 weeks. After the allergen challenge, the numbers of 
      blood eosinophils and neutrophils were examined by light microscope; the levels 
      of cytokine (interleukin (IL)-12, IL-4, tumor growth factor (TGF)-beta, IL-10, IL-6 
      and IL-17A), total immunoglobulin E (IgE) and beta-lg-specific IgE contents in the 
      serum were measured with enzyme-linked immunosorbent assay (ELISA); The mRNA 
      expression levels of TGF-beta, IL-17A,CD25, Foxp3, retinoic acid-related orphan 
      receptor gammat (RORgammat) and IL-10 were analyzed using real-time polymerase chain 
      reaction (PCR). The results showed that oral administration of L. acidophilus 
      suppressed hypersensitivity responses, attenuated the numbers of in fl ammatory 
      cells and inhibited IgE production. We found up-regulation of TGF-beta and 
      down-regulation of IL-17A in the serum of L. acidophilus-treated group, along 
      with IL-6 levels was significantly decreased than that of the allergy group 
      (p<0.05). Moreover, the mRNA expression levels of CD25, forkhead box P3 and TGF-beta 
      were significantly higher in the spleen of L. acidophilus-treated group, while 
      the mRNA expression levels of IL-17A, RORgammat and IL-10 were significantly lower 
      than that in the allergy group (p<0.05). In conclusion, the suppression of major 
      allergic symptoms by oral administration of L.acidophilus was probably due to 
      improve the regulatory T (Treg)/Th17 balance and inhibit the IL-6 production.
FAU - Li, Ai-Li
AU  - Li AL
AD  - Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, 
      Northeast Agriculture University, 59 Mucai Street, Xiangfang District, Harbin, 
      Heilongjiang 150030, China.
FAU - Meng, Xiang-Chen
AU  - Meng XC
FAU - Duan, Cui-Cui
AU  - Duan CC
FAU - Huo, Gui-Cheng
AU  - Huo GC
FAU - Zheng, Quan-Ling
AU  - Zheng QL
FAU - Li, Dan
AU  - Li D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121203
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Allergens)
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - 0 (Lactoglobulins)
RN  - 0 (Nuclear Receptor Subfamily 1, Group F, Member 3)
RN  - 0 (Rorc protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Administration, Oral
MH  - Allergens/*adverse effects
MH  - Animals
MH  - Anti-Allergic Agents/*administration & dosage
MH  - Cattle
MH  - Cytokines/blood/genetics
MH  - Female
MH  - Gene Expression
MH  - Hot Temperature
MH  - Immunoglobulin E/blood
MH  - Interleukin-2 Receptor alpha Subunit/genetics
MH  - *Lactobacillus acidophilus
MH  - Lactoglobulins/*adverse effects
MH  - Leukocyte Count
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Milk Hypersensitivity/etiology/immunology/*therapy
MH  - Nuclear Receptor Subfamily 1, Group F, Member 3/genetics
MH  - Th17 Cells/immunology
MH  - Transforming Growth Factor beta/genetics
EDAT- 2012/12/05 06:00
MHDA- 2013/08/06 06:00
CRDT- 2012/12/05 06:00
PHST- 2012/12/05 06:00 [entrez]
PHST- 2012/12/05 06:00 [pubmed]
PHST- 2013/08/06 06:00 [medline]
AID - DN/JST.JSTAGE/bpb/b12-00437 [pii]
AID - 10.1248/bpb.b12-00437 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2013;36(2):202-7. doi: 10.1248/bpb.b12-00437. Epub 2012 Dec 3.