PMID- 23209128
OWN - NLM
STAT- MEDLINE
DCOM- 20130724
LR  - 20220317
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 161
IP  - 2
DP  - 2013 Feb
TI  - High light acclimation in the secondary plastids containing diatom Phaeodactylum 
      tricornutum is triggered by the redox state of the plastoquinone pool.
PG  - 853-65
LID - 10.1104/pp.112.207811 [doi]
AB  - In diatoms, the process of energy-dependent chlorophyll fluorescence quenching 
      (qE) has an important role in photoprotection. Three components are essential for 
      qE: (1) the light-dependent generation of a transthylakoidal proton gradient; (2) 
      the deepoxidation of the xanthophyll diadinoxanthin (Dd) into diatoxanthin (Dt); 
      and (3) specific nucleus-encoded antenna proteins, called Light Harvesting 
      Complex Protein X (LHCX). We used the model diatom Phaeodactylum tricornutum to 
      investigate the concerted light acclimation response of the qE key components 
      LHCX, proton gradient, and xanthophyll cycle pigments (Dd+Dt) and to identify the 
      intracellular light-responsive trigger. At high-light exposure, the up-regulation 
      of three of the LHCX genes and the de novo synthesis of Dd+Dt led to a pronounced 
      rise of qE. By inhibiting either the conversion of Dd to Dt or the translation of 
      LHCX genes, qE amplification was abolished and the diatom cells suffered from 
      stronger photoinhibition. Artificial modification of the redox state of the 
      plastoquinone (PQ) pool via 3-(3,4-dichlorophenyl)-1,1-dimethylurea and 
      5-dibromo-6-isopropyl-3-methyl-1,4-benzoquinone resulted in a disturbance of 
      Dd+Dt synthesis in an opposite way. Moreover, we could increase the transcription 
      of two of the four LHCX genes under low-light conditions by reducing the PQ pool 
      using 5-dibromo-6-isopropyl-3-methyl-1,4-benzoquinone. Altogether, our results 
      underline the central role of the redox state of the PQ pool in the light 
      acclimation of diatoms. Additionally, they emphasize strong evidence for the 
      existence of a plastid-to-nucleus retrograde signaling mechanism in an organism 
      with plastids that derived from secondary endosymbiosis.
FAU - Lepetit, Bernard
AU  - Lepetit B
AD  - Littoral Environnement et Societes, Unite Mixte de Recherche 7266, Centre 
      National de la Recherche Scientifique-University of La Rochelle, Institute for 
      Coastal and Environmental Research, 17000 La Rochelle, France. 
      bernard.lepetit@univ-lr.fr
FAU - Sturm, Sabine
AU  - Sturm S
FAU - Rogato, Alessandra
AU  - Rogato A
FAU - Gruber, Ansgar
AU  - Gruber A
FAU - Sachse, Matthias
AU  - Sachse M
FAU - Falciatore, Angela
AU  - Falciatore A
FAU - Kroth, Peter G
AU  - Kroth PG
FAU - Lavaud, Johann
AU  - Lavaud J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121203
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Benzoquinones)
RN  - 0 (Herbicides)
RN  - 0 (Light-Harvesting Protein Complexes)
RN  - 0 (Protein Isoforms)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Xanthophylls)
RN  - 1406-65-1 (Chlorophyll)
RN  - 98600C0908 (Cycloheximide)
RN  - 9I3SDS92WY (Diuron)
RN  - OAC30J69CN (Plastoquinone)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Acclimatization/drug effects/*radiation effects
MH  - Benzoquinones/pharmacology
MH  - Blotting, Western
MH  - Chlorophyll/chemistry/metabolism
MH  - Cycloheximide/pharmacology
MH  - Diatoms/genetics/*metabolism
MH  - Dithiothreitol/pharmacology
MH  - Diuron/pharmacology
MH  - Fluorescence
MH  - Gene Expression/drug effects/radiation effects
MH  - Herbicides/pharmacology
MH  - *Light
MH  - Light-Harvesting Protein Complexes/genetics/metabolism
MH  - Oxidation-Reduction/drug effects/radiation effects
MH  - Plastids/genetics/*metabolism
MH  - Plastoquinone/*metabolism
MH  - Protein Isoforms/genetics/metabolism
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Xanthophylls/metabolism
PMC - PMC3561024
EDAT- 2012/12/05 06:00
MHDA- 2013/07/25 06:00
PMCR- 2012/12/03
CRDT- 2012/12/05 06:00
PHST- 2012/12/05 06:00 [entrez]
PHST- 2012/12/05 06:00 [pubmed]
PHST- 2013/07/25 06:00 [medline]
PHST- 2012/12/03 00:00 [pmc-release]
AID - pp.112.207811 [pii]
AID - 207811 [pii]
AID - 10.1104/pp.112.207811 [doi]
PST - ppublish
SO  - Plant Physiol. 2013 Feb;161(2):853-65. doi: 10.1104/pp.112.207811. Epub 2012 Dec 
      3.