PMID- 23211755
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20231213
IS  - 1424-8220 (Electronic)
IS  - 1424-8220 (Linking)
VI  - 12
IP  - 12
DP  - 2012 Dec 4
TI  - Development of a one-step immunocapture real-time RT-PCR assay for detection of 
      tobacco mosaic virus in soil.
PG  - 16685-94
LID - 10.3390/s121216685 [doi]
AB  - Tobacco mosaic virus (TMV) causes significant losses in many economically 
      important crops. Contaminated soils may play roles as reservoirs and sources of 
      transmission for TMV. In this study we report the development of an immunocapture 
      real-time RT-PCR (IC-real-time RT-PCR) assay for direct detection of TMV in soils 
      without RNA isolation. A series of TMV infected leaf sap dilutions of 1:101, 
      1:102, 1:103, 1:104, 1:105 and 1:106 (w/v, g/mL) were added to one gram of soil. 
      The reactivity of DAS-ELISA and conventional RT-PCR was in the range of 1:102 and 
      1:103 dilution in TMV-infested soils, respectively. Meanwhile, the detection 
      limit of IC-real-time RT-PCR sensitivity was up to 1:106 dilution. However, in 
      plant sap infected by TMV, both IC-real-time RT-PCR and real-time RT-PCR were up 
      to 1:106 dilution, DAS-ELISA could detect at least 1:103 dilution. IC-real-time 
      RT-PCR method can use either plant sample extracts or cultivated soils, and show 
      higher sensitivity than RT-PCR and DAS-ELISA for detection of TMV in soils. 
      Therefore, the proposed IC-real-time RT-PCR assay provides an alternative for 
      quick and very sensitive detection of TMV in soils, with the advantage of not 
      requiring a concentration or RNA purification steps while still allowing 
      detection of TMV for disease control.
FAU - Yang, Jin-Guang
AU  - Yang JG
AD  - Open Project Program of Key Laboratory of Tobacco Pest Monitoring Controlling & 
      Integrated Management, Tobacco Research Institute of CAAS, Qingdao 266101, China. 
      jinguangyang@126.com
FAU - Wang, Feng-Long
AU  - Wang FL
FAU - Chen, De-Xin
AU  - Chen DX
FAU - Shen, Li-Li
AU  - Shen LL
FAU - Qian, Yu-Mei
AU  - Qian YM
FAU - Liang, Zhi-Yong
AU  - Liang ZY
FAU - Zhou, Wen-Chang
AU  - Zhou WC
FAU - Yan, Tai-He
AU  - Yan TH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121204
PL  - Switzerland
TA  - Sensors (Basel)
JT  - Sensors (Basel, Switzerland)
JID - 101204366
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Plant Leaves/virology
MH  - RNA, Viral
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - *Soil Microbiology
MH  - Nicotiana/virology
MH  - Tobacco Mosaic Virus/*isolation & purification
PMC - PMC3571805
EDAT- 2012/12/06 06:00
MHDA- 2014/01/01 06:00
PMCR- 2012/12/01
CRDT- 2012/12/06 06:00
PHST- 2012/10/30 00:00 [received]
PHST- 2012/11/13 00:00 [revised]
PHST- 2012/11/14 00:00 [accepted]
PHST- 2012/12/06 06:00 [entrez]
PHST- 2012/12/06 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
AID - s121216685 [pii]
AID - sensors-12-16685 [pii]
AID - 10.3390/s121216685 [doi]
PST - epublish
SO  - Sensors (Basel). 2012 Dec 4;12(12):16685-94. doi: 10.3390/s121216685.