PMID- 23211962
OWN - NLM
STAT- MEDLINE
DCOM- 20130325
LR  - 20211021
IS  - 1559-7016 (Electronic)
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 33
IP  - 2
DP  - 2013 Feb
TI  - CART peptide induces neuroregeneration in stroke rats.
PG  - 300-10
LID - 10.1038/jcbfm.2012.172 [doi]
AB  - Utilizing a classic stroke model in rodents, middle cerebral artery occlusion 
      (MCAo), we describe a novel neuroregenerative approach using the repeated 
      intranasal administration of cocaine- and amphetamine-regulated transcript (CART) 
      peptide starting from day 3 poststroke for enhancing the functional recovery of 
      injured brain. Adult rats were separated into two groups with similar infarction 
      sizes, measured by magnetic resonance imaging on day 2 after MCAo, and were 
      treated with CART or vehicle. The CART treatment increased CART level in the 
      brain, improved behavioral recovery, and reduced neurological scores. In the 
      subventricular zone (SVZ), CART enhanced immunolabeling of bromodeoxyuridine, a 
      neural progenitor cell marker Musashi-1, and the proliferating cell nuclear 
      antigen, as well as upregulated brain-derived neurotrophic factor (BDNF) mRNA. 
      AAV-GFP was locally applied to the SVZ to examine migration of SVZ cells. The 
      CART enhanced migration of GFP(+) cells from SVZ toward the ischemic cortex. In 
      SVZ culture, CART increased the size of neurospheres. The CART-mediated cell 
      migration from SVZ explants was reduced by anti-BDNF blocking antibody. Using 
      (1)H-MRS (proton magnetic resonance spectroscopy), increases in N-acetylaspartate 
      levels were found in the lesioned cortex after CART treatment in stroke brain. 
      Cocaine- and amphetamine-regulated transcript increased the expression of GAP43 
      and fluoro-ruby fluorescence in the lesioned cortex. In conclusion, our data 
      suggest that intranasal CART treatment facilitates neuroregeneration in stroke 
      brain.
FAU - Luo, Yu
AU  - Luo Y
AD  - National Institute on Drug Abuse, Intramural Research Program, Baltimore, 
      Maryland 21224, USA.
FAU - Shen, Hui
AU  - Shen H
FAU - Liu, Hua-Shan
AU  - Liu HS
FAU - Yu, Seong-Jin
AU  - Yu SJ
FAU - Reiner, David J
AU  - Reiner DJ
FAU - Harvey, Brandon K
AU  - Harvey BK
FAU - Hoffer, Barry J
AU  - Hoffer BJ
FAU - Yang, Yihong
AU  - Yang Y
FAU - Wang, Yun
AU  - Wang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20121205
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Msi1 protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (cocaine- and amphetamine-regulated transcript protein)
SB  - IM
MH  - Administration, Intranasal
MH  - Animals
MH  - Behavior, Animal/drug effects
MH  - Brain-Derived Neurotrophic Factor/biosynthesis
MH  - Cell Movement/drug effects
MH  - Cerebral Cortex/metabolism/pathology/physiopathology
MH  - Disease Models, Animal
MH  - GAP-43 Protein/biosynthesis
MH  - Male
MH  - Nerve Tissue Proteins/metabolism/*toxicity
MH  - Neural Stem Cells/metabolism/pathology
MH  - Neurodegenerative Diseases/chemically 
      induced/*metabolism/pathology/physiopathology
MH  - RNA, Messenger/biosynthesis
MH  - RNA-Binding Proteins/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stroke/complications/*metabolism/pathology/physiopathology
MH  - Time Factors
MH  - Up-Regulation/drug effects
PMC - PMC3564201
EDAT- 2012/12/06 06:00
MHDA- 2013/03/26 06:00
PMCR- 2013/02/01
CRDT- 2012/12/06 06:00
PHST- 2012/12/06 06:00 [entrez]
PHST- 2012/12/06 06:00 [pubmed]
PHST- 2013/03/26 06:00 [medline]
PHST- 2013/02/01 00:00 [pmc-release]
AID - jcbfm2012172 [pii]
AID - 10.1038/jcbfm.2012.172 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 2013 Feb;33(2):300-10. doi: 10.1038/jcbfm.2012.172. 
      Epub 2012 Dec 5.