PMID- 23212569 OWN - NLM STAT- MEDLINE DCOM- 20141007 LR - 20220409 IS - 1993-0402 (Electronic) IS - 1672-0415 (Linking) VI - 20 IP - 1 DP - 2014 Jan TI - Effects of Zuogui Pill () and Yougui Pill () on the expression of brain-derived neurotrophic factor and cyclic adenosine monophosphate/protein kinase A signaling transduction pathways of axonal regeneration in model rats with experimental autoimmune encephalomyelitis. PG - 24-30 LID - 10.1007/s11655-012-1236-2 [doi] AB - OBJECTIVE: To study the effects of Zuogui Pill (, ZGP) and Yougui Pill (, YGP) on the expressions of brain-derived neurotrophic factor (BDNF) and cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) signaling of axonal regeneration in the Lewis rats with experimental autoimmune encephalomyelitis (EAE), in order to explore the possible mechanism of ZGP and YGP on promoting axonal regeneration. METHODS: The rats were randomly divided into normal control (NC), model (MO), prednisone acetate (PA), ZGP and YGP groups. The EAE model of rat was established by injecting antigen containing myelin basic protein (MBP)68-86. The brain and spinal cord were harvested on the 14th and 28th day post-immunization (PI), the protein and mRNA expression of BDNF and PKA in the brain and spinal cord of rats were detected by Western blot analysis and real-time quantitative polymerase chain reaction (PCR), and the cAMP levels were detected by using enzyme-immunoassay method. RESULTS: (1) On the 28th day PI, the mRNA expression of BDNF in brain white matter and spinal cord of rats in ZGP and YGP groups were up-regulated, especially in YGP group (P<0.05 or P<0.01). (2) On the 14th day PI, the cAMP levels in brain white matters significantly increased in PA and YGP groups compared with MO group (P<0.05 or P<0.01), and the cAMP level in YGP group was higher than that in ZGP group (P<0.05). The cAMP level in spinal cord also significantly increased in YGP group compared with MO, PA and ZGP groups, respectively (P<0.01). (3) On the 14th day PI, the PKA expression in spinal cord of rats in ZGP group was significantly decreased compared with MO and YGP groups, respectively (P<0.05). (4) On the 28th day PI, there was a positive correlation between cAMP and PKA expression in the brain white matter of YGP rats. CONCLUSIONS: The results suggest that ZGP and YGP may promote axonal regeneration by modulating cAMP/PKA signal transduction pathway, but the targets of molecular mechanism of ZGP may be different from those of YGP. FAU - Wang, Yi-zhou AU - Wang YZ AD - School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China. FAU - Kou, Shuang AU - Kou S FAU - Gu, Ling-yun AU - Gu LY FAU - Zheng, Qi AU - Zheng Q FAU - Li, Ming AU - Li M FAU - Qi, Fang AU - Qi F FAU - Zhao, Hui AU - Zhao H FAU - Wang, Lei AU - Wang L LA - eng PT - Journal Article DEP - 20121203 PL - China TA - Chin J Integr Med JT - Chinese journal of integrative medicine JID - 101181180 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Drugs, Chinese Herbal) RN - 0 (RNA, Messenger) RN - 0 (Tablets) RN - 0 (yougui) RN - 0 (zuogui) RN - E0399OZS9N (Cyclic AMP) RN - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases) SB - IM MH - Animals MH - Axons/drug effects/pathology MH - Brain/drug effects/metabolism/pathology MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Cyclic AMP/*metabolism MH - Cyclic AMP-Dependent Protein Kinases/genetics/*metabolism MH - Disease Models, Animal MH - Drugs, Chinese Herbal/pharmacology/*therapeutic use MH - Encephalomyelitis, Autoimmune, Experimental/*drug therapy/enzymology/genetics MH - Female MH - Gene Expression Regulation/drug effects MH - *Nerve Regeneration/drug effects/genetics MH - RNA, Messenger/genetics/metabolism MH - Rats MH - Rats, Inbred Lew MH - Signal Transduction/drug effects/genetics MH - Spinal Cord/drug effects/metabolism/pathology MH - Tablets EDAT- 2012/12/06 06:00 MHDA- 2014/10/08 06:00 CRDT- 2012/12/06 06:00 PHST- 2011/06/04 00:00 [received] PHST- 2012/12/06 06:00 [entrez] PHST- 2012/12/06 06:00 [pubmed] PHST- 2014/10/08 06:00 [medline] AID - 10.1007/s11655-012-1236-2 [doi] PST - ppublish SO - Chin J Integr Med. 2014 Jan;20(1):24-30. doi: 10.1007/s11655-012-1236-2. Epub 2012 Dec 3.