PMID- 23213272
OWN - NLM
STAT- MEDLINE
DCOM- 20130514
LR  - 20211021
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 18
DP  - 2012
TI  - Nectin-1-specific entry of herpes simplex virus 1 is sufficient for infection of 
      the cornea and viral spread to the trigeminal ganglia.
PG  - 2711-6
AB  - PURPOSE: Primary and recurrent infections of the cornea by herpes simplex virus 1 
      (HSV-1) are important causes of eye disease. Three unrelated classes of 
      glycoprotein D receptors for HSV-1 entry into cells have been identified. This 
      study was undertaken to uncover the relative significance of nectin-1 as an entry 
      receptor in corneal infection and HSV-1 spread to the trigeminal ganglia (TG), a 
      site important for HSV-1 latency and recurrent corneal infection. METHODS: To 
      assess the significance of nectin-1, a member of the immunoglobulin superfamily, 
      in primary HSV-1 infection and spread to the TG, we used a murine model of 
      corneal infection and a HSV-1 mutant, KOS(Rid1), which can only use nectin-1 for 
      entry. Immunohistochemistry, real-time PCR, and plaque assays using HSV-1 
      infected tissues were performed. RESULTS: We demonstrated that receptor usage by 
      HSV-1 limited to nectin-1 does not significantly change the spread of HSV-1 in 
      the corneal epithelium during primary infection. We also found that 
      nectin-1-specific entry does not affect the capacity of the virus to spread to 
      the TG from the cornea. CONCLUSIONS: Our findings suggest that nectin-1 alone is 
      sufficient for HSV-1 entry into the cornea and spread to the TG.
FAU - Shukla, Navika D
AU  - Shukla ND
AD  - Department of Pathology, College of Medicine, University of Illinois at Chicago, 
      Chicago, IL 60612, USA.
FAU - Tiwari, Vaibhav
AU  - Tiwari V
FAU - Valyi-Nagy, Tibor
AU  - Valyi-Nagy T
LA  - eng
PT  - Journal Article
DEP - 20121116
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (NECTIN1 protein, human)
RN  - 0 (Nectin1 protein, mouse)
RN  - 0 (Nectins)
RN  - 0 (Receptors, Virus)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules/genetics/*metabolism
MH  - Disease Models, Animal
MH  - Epithelium, Corneal/*metabolism/pathology/virology
MH  - Gene Expression
MH  - Herpes Simplex/*metabolism/pathology/virology
MH  - Herpesvirus 1, Human/genetics/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratitis, Herpetic
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mutation
MH  - Nectins
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Virus/genetics/*metabolism
MH  - Trigeminal Ganglion/*metabolism/pathology/virology
MH  - Viral Plaque Assay
MH  - *Virus Internalization
MH  - Virus Latency
MH  - Virus Replication
PMC - PMC3513192
EDAT- 2012/12/06 06:00
MHDA- 2013/05/15 06:00
PMCR- 2012/01/01
CRDT- 2012/12/06 06:00
PHST- 2012/08/23 00:00 [received]
PHST- 2012/11/14 00:00 [accepted]
PHST- 2012/12/06 06:00 [entrez]
PHST- 2012/12/06 06:00 [pubmed]
PHST- 2013/05/15 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - 278 [pii]
PST - ppublish
SO  - Mol Vis. 2012;18:2711-6. Epub 2012 Nov 16.