PMID- 23213352
OWN - NLM
STAT- MEDLINE
DCOM- 20130403
LR  - 20230429
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2012
DP  - 2012
TI  - Group VIB Phospholipase A(2) promotes proliferation of INS-1 insulinoma cells and 
      attenuates lipid peroxidation and apoptosis induced by inflammatory cytokines and 
      oxidant agents.
PG  - 989372
LID - 10.1155/2012/989372 [doi]
LID - 989372
AB  - Group VIB Phospholipase A(2) (iPLA(2)gamma) is distributed in membranous organelles 
      in which beta-oxidation occurs, that is, mitochondria and peroxisomes, and is 
      expressed by insulin-secreting pancreatic islet beta-cells and INS-1 insulinoma 
      cells, which can be injured by inflammatory cytokines, for example, IL-1beta and 
      IFN-gamma, and by oxidants, for example, streptozotocin (STZ) or 
      t-butyl-hydroperoxide (TBHP), via processes pertinent to mechanisms of beta-cell 
      loss in types 1 and 2 diabetes mellitus. We find that incubating INS-1 cells with 
      IL-1beta and IFN-gamma, with STZ, or with TBHP causes increased expression of iPLA(2)gamma 
      mRNA and protein. We prepared INS-1 knockdown (KD) cell lines with reduced 
      iPLA(2)gamma expression, and they proliferate more slowly than control INS-1 cells 
      and undergo increased membrane peroxidation in response to cytokines or oxidants. 
      Accumulation of oxidized phospholipid molecular species in STZ-treated INS-1 
      cells was demonstrated by LC/MS/MS scanning, and the levels in iPLA(2)gamma-KD cells 
      exceeded those in control cells. iPLA(2)gamma-KD INS-1 cells also exhibited higher 
      levels of apoptosis than control cells when incubated with STZ or with IL-1beta and 
      IFN-gamma. These findings suggest that iPLA(2)gamma promotes beta-cell proliferation and 
      that its expression is increased during inflammation or oxidative stress as a 
      mechanism to mitigate membrane injury that may enhance beta-cell survival.
FAU - Bao, Shunzhong
AU  - Bao S
AD  - Mass Spectrometry Resource, Division of Endocrinology, Metabolism, and Lipid 
      Research, Department of Medicine, Washington University School of Medicine, 
      Campus Box 8127, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
FAU - Song, Haowei
AU  - Song H
FAU - Tan, Min
AU  - Tan M
FAU - Wohltmann, Mary
AU  - Wohltmann M
FAU - Ladenson, Jack H
AU  - Ladenson JH
FAU - Turk, John
AU  - Turk J
LA  - eng
GR  - R37 DK034388/DK/NIDDK NIH HHS/United States
GR  - P60-DK20579/DK/NIDDK NIH HHS/United States
GR  - P30 DK056341/DK/NIDDK NIH HHS/United States
GR  - 5 T32 DK007120/DK/NIDDK NIH HHS/United States
GR  - R37-DK34388/DK/NIDDK NIH HHS/United States
GR  - T32 DK007120/DK/NIDDK NIH HHS/United States
GR  - P60 DK020579/DK/NIDDK NIH HHS/United States
GR  - P30 DK020579/DK/NIDDK NIH HHS/United States
GR  - P41 RR000954/RR/NCRR NIH HHS/United States
GR  - P41-RR00954/RR/NCRR NIH HHS/United States
GR  - P30-DK56341/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20121111
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipids)
RN  - 0 (Oxidants)
RN  - 5W494URQ81 (Streptozocin)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 955VYL842B (tert-Butylhydroperoxide)
RN  - EC 3.1.1.4 (Group VI Phospholipases A2)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Chromatography, High Pressure Liquid
MH  - Cytokines/*pharmacology
MH  - Gene Knockdown Techniques
MH  - Group VI Phospholipases A2/*metabolism
MH  - Inflammation Mediators/pharmacology
MH  - Insulinoma/*enzymology/*pathology
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-1beta/pharmacology
MH  - Lipid Peroxidation/*drug effects
MH  - Lipids/chemistry
MH  - Oxidants/*pharmacology
MH  - Oxidation-Reduction/drug effects
MH  - Rats
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Streptozocin/pharmacology
MH  - tert-Butylhydroperoxide/pharmacology
PMC - PMC3503447
EDAT- 2012/12/06 06:00
MHDA- 2013/04/04 06:00
PMCR- 2012/11/11
CRDT- 2012/12/06 06:00
PHST- 2012/06/27 00:00 [received]
PHST- 2012/08/20 00:00 [accepted]
PHST- 2012/12/06 06:00 [entrez]
PHST- 2012/12/06 06:00 [pubmed]
PHST- 2013/04/04 06:00 [medline]
PHST- 2012/11/11 00:00 [pmc-release]
AID - 10.1155/2012/989372 [doi]
PST - ppublish
SO  - Oxid Med Cell Longev. 2012;2012:989372. doi: 10.1155/2012/989372. Epub 2012 Nov 
      11.