PMID- 23213783
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20121206
IS  - 1004-5619 (Print)
IS  - 1004-5619 (Linking)
VI  - 28
IP  - 5
DP  - 2012 Oct
TI  - [Freeze grinding combined ultrasonic technique for the treatment of nail test 
      material].
PG  - 342-6
AB  - OBJECTIVE: To investigate the feasibility of the new method of combining freeze 
      grinding with ultrasonic technique for the pretreatment of the nail for 
      toxicological and pharmaceutical analysis and to compare the advantages and 
      disadvantages of this method with other traditional methods. METHODS: Five 
      pretreatment methods were examined. Scanning electron microscope (SEM) was used 
      to observe the microstructural changes of the nail. RESULTS: The microscopic 
      structure of nail totally destroyed after alkali treatment. The hierarchy mode of 
      the internal structure became obvious and tight after acid hydrolysis, which 
      became indistinct after methanol infiltration. The structure of nail broke to 
      pieces after ultrasonic technique. After freeze grinding combined ultrasonic 
      technique, the particle structure kept original shape, and its size was one 
      hundred times smaller than which after manual way. CONCLUSION: The freeze 
      grinding combined ultrasonic technique can improve the release efficiency, and 
      ensure the stability of the toxicant or drug during the pretreatment process. It 
      is appropriate for toxicological and pharmaceutical analysis in the nail.
FAU - Chen, Hang
AU  - Chen H
AD  - Department of Forensic Medicine, Shanghai Medical College, Fudan University, 
      Shanghai 200032, China. Dio.Chen.Hang@gmail.com
FAU - Xiang, Ping
AU  - Xiang P
FAU - Sun, Qi-ran
AU  - Sun QR
FAU - Shen, Min
AU  - Shen M
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Fa Yi Xue Za Zhi
JT  - Fa yi xue za zhi
JID - 9426151
SB  - IM
MH  - Forensic Pathology
MH  - Freeze Drying/*methods
MH  - Humans
MH  - *Microscopy, Electron, Scanning
MH  - Nails/*chemistry
MH  - Particle Size
MH  - *Ultrasonics
EDAT- 2012/12/12 06:00
MHDA- 2015/05/15 06:00
CRDT- 2012/12/11 06:00
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
PST - ppublish
SO  - Fa Yi Xue Za Zhi. 2012 Oct;28(5):342-6.