PMID- 23217174
OWN - NLM
STAT- MEDLINE
DCOM- 20141023
LR  - 20181202
IS  - 1865-1682 (Electronic)
IS  - 1865-1674 (Linking)
VI  - 61
IP  - 4
DP  - 2014 Aug
TI  - Development of indirect ELISAs for differential serodiagnosis of classical and 
      highly pathogenic porcine reproductive and respiratory syndrome virus.
PG  - 341-9
LID - 10.1111/tbed.12040 [doi]
AB  - The objective of this study was to develop two indirect enzyme-linked 
      immunosorbent assays (iELISAs) for detection of serum antibodies against 
      classical vaccine strain of porcine reproductive and respiratory syndrome virus 
      (PRRSV) and highly pathogenic PRRSV (HP-PRRSV). To detect the common antibodies 
      against classical and HP-PRRSV, the coating antigen used in the iELISA 
      (designated iELISA-180) was the antigen of Nsp2-180, the 180aa at amino terminal 
      of Nsp2. To detect the different antibodies against classical and HP-PRRSV, the 
      coating antigen in the second iELISA (designated iELISA-D29) was Nsp2-D29, the 
      deleted 29aa in Nsp2 of HP-PRRSV. The antigen concentration and serum dilutions 
      were optimized using a draughtboard titration. The cut-off values of 0.361 at 
      OD(450nm) for the iELISA-180 and 0.27 at OD(450nm) for the iELISA-D29 were 
      determined by testing a panel of 120 classical PRRSV positive and 198 PRRSV 
      negative pig serum samples, which generated the specificity of 97.1% and 96.7%, 
      the sensitivity of 96.9% and 96.3% for iELISA-180 and iELISA-D29, respectively. 
      The agreements between the Western blot and iELISA-180 and iELISA-D29 were 98%, 
      96.7%, respectively. The developed iELISAs can be used to differentiate 
      serologically HP-PRRSV from the vaccinated or classical PRRSV in clinical serum 
      samples.
CI  - (c) 2012 Blackwell Verlag GmbH.
FAU - Xiao, Y H
AU  - Xiao YH
AD  - Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong 
      Agricultural University, Shandong, China.
FAU - Wang, T T
AU  - Wang TT
FAU - Zhao, Q
AU  - Zhao Q
FAU - Wang, C B
AU  - Wang CB
FAU - Lv, J H
AU  - Lv JH
FAU - Nie, L
AU  - Nie L
FAU - Gao, J M
AU  - Gao JM
FAU - Ma, X C
AU  - Ma XC
FAU - Hsu, W H
AU  - Hsu WH
FAU - Zhou, E M
AU  - Zhou EM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121210
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Antigens, Viral/*immunology
MH  - Blotting, Western
MH  - Diagnosis, Differential
MH  - Enzyme-Linked Immunosorbent Assay/methods/*veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*diagnosis/prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/isolation & 
      purification/pathogenicity
MH  - Recombinant Proteins/immunology
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Viral Vaccines/immunology
OTO - NOTNLM
OT  - ELISA
OT  - Nsp2
OT  - PRRSV
OT  - diagnosis
OT  - highly pathogenic PRRSV
EDAT- 2012/12/12 06:00
MHDA- 2014/10/24 06:00
CRDT- 2012/12/11 06:00
PHST- 2012/10/06 00:00 [received]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2014/10/24 06:00 [medline]
AID - 10.1111/tbed.12040 [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2014 Aug;61(4):341-9. doi: 10.1111/tbed.12040. Epub 2012 
      Dec 10.