PMID- 23217298
OWN - NLM
STAT- MEDLINE
DCOM- 20130429
LR  - 20131121
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 911
DP  - 2012 Dec 12
TI  - Highly sensitive trivalent copper chelate-luminol chemiluminescence system for 
      capillary electrophoresis detection of epinephrine in the urine of smoker.
PG  - 1-5
LID - S1570-0232(12)00601-0 [pii]
LID - 10.1016/j.jchromb.2012.10.017 [doi]
AB  - Epinephrine (EP) is one of the most important neurotransmitters and hormones. 
      Some previous literatures show that there is a close relation between its release 
      and smoking. To compare the levels of EP in urines of smokers and nonsmokers, a 
      sensitive chemiluminescence (CL) system, luminol-diperiodatocuprate (III) 
      (K(5)[Cu(HIO(6))(2)], DPC), has been developed and validated for the 
      determination of EP after CE separation. The DPC-luminol-EP CL reaction showed 
      very intensive emission and fast kinetic characteristics, thus led to a high 
      sensitivity in the flow-through detection mode for capillary electrophoresis. 
      With the peak height as a quantitative parameter, the relative CL intensity was 
      linear with the EP concentration in the range of 2.0-400ng/mL, with a limit of 
      detection of 0.82ng/mL (S/N=3). The reproducibility was assessed by intra- and 
      inter-day relative standard deviations (RSDs) for 11 replicate determinations of 
      EP standard samples at low, medium and high concentrations. The intra- and 
      inter-day RSDs for CL signals were 5.5%-6.6% and 6.1%-7.5%, respectively, and 
      those for migration times were 3.4%-5.8% and 4.3%-6.3%, respectively. The 
      presented method was successfully applied to the determination of EP in EP 
      injection and urine samples of smokers and nonsmokers. The recovery test results 
      for urine samples ranged from 86.5 to 112.0%, which demonstrated the reliability 
      of this method. The results for urine sample detection indicate that the average 
      level of EP in the urines of the smoker group is obviously higher than that in 
      the urines of the nonsmoker group, which may demonstrate that smoking can 
      stimulate the release of EP in human body.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Li, Tao
AU  - Li T
AD  - Key Laboratory of Luminescence and Real-Time Analysis (Ministry of Education), 
      College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, 
      China.
FAU - Wang, Zuorong
AU  - Wang Z
FAU - Xie, Haoyue
AU  - Xie H
FAU - Fu, Zhifeng
AU  - Fu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121023
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Iodates)
RN  - 0 (potassium diperiodatocuprate(III))
RN  - 5EXP385Q4F (Luminol)
RN  - 789U1901C5 (Copper)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Adult
MH  - Chromatography, High Pressure Liquid
MH  - Copper/*chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Epinephrine/*urine
MH  - Humans
MH  - Iodates/*chemistry
MH  - Luminescence
MH  - Luminescent Measurements/*methods
MH  - Luminol/*chemistry
MH  - Male
MH  - Smoking/*urine
EDAT- 2012/12/12 06:00
MHDA- 2013/04/30 06:00
CRDT- 2012/12/11 06:00
PHST- 2012/06/16 00:00 [received]
PHST- 2012/09/11 00:00 [revised]
PHST- 2012/10/15 00:00 [accepted]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/04/30 06:00 [medline]
AID - S1570-0232(12)00601-0 [pii]
AID - 10.1016/j.jchromb.2012.10.017 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Dec 12;911:1-5. doi: 
      10.1016/j.jchromb.2012.10.017. Epub 2012 Oct 23.