PMID- 23219026
OWN - NLM
STAT- MEDLINE
DCOM- 20130520
LR  - 20171116
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1271
IP  - 1
DP  - 2013 Jan 4
TI  - Preparation of phenylboronic acid-silica hybrid monolithic column with one-pot 
      approach for capillary liquid chromatography of biomolecules.
PG  - 115-23
LID - S0021-9673(12)01769-4 [pii]
LID - 10.1016/j.chroma.2012.11.038 [doi]
AB  - A phenylboronic acid-silica hybrid monolithic column for capillary liquid 
      chromatography (cLC) was prepared through one-pot process by using 
      4-vinylphenylboronic acid (VPBA) and alkoxysilanes simultaneously. The effects of 
      the molar ratio of tetramethyloxysilane/gamma-methacryloxypropyltrimethoxysilane 
      (TMOS/gamma-MAPS), amount of VPBA, and the volume of diethylene glycol (DEG) on the 
      morphologies, permeabilities and pore properties of the prepared VPBA-silica 
      hybrid monolithic columns were studied in detail. A relatively uniform monolithic 
      structure with high porosity was obtained with optimized ingredients. A series of 
      cis-diol-containing compounds, alkylbenzenes, amides, and anilines were utilized 
      to evaluate the retention behaviors of the VPBA-silica hybrid monolithic column. 
      The result demonstrated that the prepared VPBA-silica hybrid monolithic column 
      exhibited multiple interactions including hydrophobicity, hydrophilicity, as well 
      as cation exchange apart from the expected affinity interaction. The run-to-run, 
      column-to-column and batch-to-batch reproducibility of the VPBA-silica hybrid 
      monolith were satisfactory with the relative standard deviations (RSDs) less than 
      1.63% (n=5), 2.02% (n=3) and 2.90% (n=5), respectively, indicating the 
      effectiveness and practicability of the proposed method. In addition, the 
      VPBA-silica hybrid monolithic column was further applied to the separation of 
      proteins and tryptic digest of bovine serum albumin (BSA), respectively. The 
      successful applications suggested the potential of the VPBA-silica hybrid 
      monolith in proteome analysis.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Lin, Zian
AU  - Lin Z
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. 
      zianlin@fzu.edu.cn
FAU - Huang, Hui
AU  - Huang H
FAU - Li, Shihua
AU  - Li S
FAU - Wang, Juan
AU  - Wang J
FAU - Tan, Xiaoqing
AU  - Tan X
FAU - Zhang, Lan
AU  - Zhang L
FAU - Chen, Guonan
AU  - Chen G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121121
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (4-vinylphenyl boronic acid)
RN  - 0 (Acetonitriles)
RN  - 0 (Benzene Derivatives)
RN  - 0 (Boronic Acids)
RN  - 0 (Peptide Fragments)
RN  - 0 (Vinyl Compounds)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetonitriles/chemistry
MH  - Animals
MH  - Benzene Derivatives/chemistry
MH  - Boronic Acids/*chemistry
MH  - Cattle
MH  - Chromatography, Liquid/*instrumentation/methods
MH  - Hydrogen-Ion Concentration
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Peptide Fragments/analysis/chemistry
MH  - Reproducibility of Results
MH  - Serum Albumin, Bovine/chemistry
MH  - Silicon Dioxide/*chemistry
MH  - Vinyl Compounds/*chemistry
EDAT- 2012/12/12 06:00
MHDA- 2013/05/22 06:00
CRDT- 2012/12/11 06:00
PHST- 2012/08/25 00:00 [received]
PHST- 2012/11/09 00:00 [revised]
PHST- 2012/11/15 00:00 [accepted]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/05/22 06:00 [medline]
AID - S0021-9673(12)01769-4 [pii]
AID - 10.1016/j.chroma.2012.11.038 [doi]
PST - ppublish
SO  - J Chromatogr A. 2013 Jan 4;1271(1):115-23. doi: 10.1016/j.chroma.2012.11.038. 
      Epub 2012 Nov 21.