PMID- 23219897
OWN - NLM
STAT- MEDLINE
DCOM- 20130402
LR  - 20181202
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 330
IP  - 2
DP  - 2013 Apr 28
TI  - Nuclear factor-E2 (Nrf2) is regulated through the differential activation of 
      ERK1/2 and PKC alpha/betaII by Gymnasterkoreayne B.
PG  - 225-32
LID - S0304-3835(12)00715-X [pii]
LID - 10.1016/j.canlet.2012.11.053 [doi]
AB  - Phytochemicals are well known to have cancer chemopreventive effects by induction 
      of phase II detoxification enzymes including quinone reductase (NQO-1) and 
      glutathione-S-transferases. These detoxification enzymes are commonly regulated 
      by nuclear factor-E2 (Nrf2), which is a representative antioxidant and 
      cytoprotective factor involved in cancer chemoprevention. As one of the known 
      quinone reductase (QR) inducers and Nrf2 activators, Gymnasterkoreayne B (GKB) 
      isolated from Gymnaster (Aster) koraiensis was used to elucidate the upstream 
      signalling pathway for Nrf2 regulation. In this study, we confirmed that GKB 
      significantly increases expression levels of Nrf2 in HCT116 human colon cancer 
      cells. We found the probable mechanism of upstream signalling pathways to 
      activate Nrf2 by GKB. To reveal the pathway that affects Nrf2 translocation by 
      GKB, we examined changes in various kinases in HCT116 cells treated with GKB. We 
      observed that ERK and PKC pathways are particularly involved in the activation of 
      Nrf2 by GKB, followed by translocation of Nrf2 and induction of NQO-1. These 
      results suggest that GKB induces Nrf2 translocation and expression by 
      differential regulation of ERK and PKC pathways in HCT116 cells.
CI  - Copyright (c) 2012 Elsevier Ireland Ltd. All rights reserved.
FAU - Lee, Kyung-Mi
AU  - Lee KM
AD  - Functional Food Center, Korea Institute of Science and Technology, Gangneung, 
      Republic of Korea.
FAU - Kang, Kyungsu
AU  - Kang K
FAU - Lee, Saet Byoul
AU  - Lee SB
FAU - Nho, Chu Won
AU  - Nho CW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121205
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Anticarcinogenic Agents)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (gymnasterkoreayne B)
RN  - 25067-58-7 (Polyynes)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (NQO1 protein, human)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C beta)
RN  - EC 2.7.11.13 (Protein Kinase C-alpha)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - Anticarcinogenic Agents/*pharmacology
MH  - HCT116 Cells
MH  - Humans
MH  - Mitogen-Activated Protein Kinase 1/*physiology
MH  - Mitogen-Activated Protein Kinase 3/*physiology
MH  - NAD(P)H Dehydrogenase (Quinone)/metabolism
MH  - NF-E2-Related Factor 2/*physiology
MH  - Polyynes/*pharmacology
MH  - Protein Kinase C/*physiology
MH  - Protein Kinase C beta
MH  - Protein Kinase C-alpha/*physiology
EDAT- 2012/12/12 06:00
MHDA- 2013/04/03 06:00
CRDT- 2012/12/11 06:00
PHST- 2012/08/31 00:00 [received]
PHST- 2012/11/28 00:00 [revised]
PHST- 2012/11/29 00:00 [accepted]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/04/03 06:00 [medline]
AID - S0304-3835(12)00715-X [pii]
AID - 10.1016/j.canlet.2012.11.053 [doi]
PST - ppublish
SO  - Cancer Lett. 2013 Apr 28;330(2):225-32. doi: 10.1016/j.canlet.2012.11.053. Epub 
      2012 Dec 5.