PMID- 23220338
OWN - NLM
STAT- MEDLINE
DCOM- 20130701
LR  - 20191210
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 171
IP  - 1
DP  - 2013 Jan
TI  - Generation and characterization of a porcine endometrial endothelial cell line 
      susceptible to porcine reproductive and respiratory syndrome virus.
PG  - 209-15
LID - S0168-1702(12)00460-1 [pii]
LID - 10.1016/j.virusres.2012.11.015 [doi]
AB  - Previous studies on the underlying mechanism for porcine reproductive and 
      respiratory syndrome virus (PRRSV)-induced reproductive failure have been focused 
      on the viral replication in the endothelial macrophages, and the susceptibility 
      of porcine endometrial endothelial (PEE) cells to PRRSV has not yet been 
      investigated. Therefore, in the present study we generated a PEE cell line by 
      transfection of the primary cells with a SV40 large T antigen expression vector. 
      The PEE cell line maintained the endothelial morphology with a significantly 
      faster growth rate, shorter population doubling time and higher plating 
      efficiency than the primary cells. The endothelial origination of the cell line 
      was confirmed by detection of the endothelial cell-specific markers. The PEE cell 
      line had been passed successively for 60 generations with an unlimited growth 
      potential. To further characterize the PEE cell line, cells of different passages 
      were infected with different PRRSV strains and analyzed for the viral antigen and 
      replication. Overt cytopathic effect was observed from 36h postinfection (HPI) 
      and the viral antigen detected as early as 12 HPI. The infectious virus was 
      recovered from the infected PEE cells with a titer higher than that in MARC-145 
      cells. Since the data presented indicate a high susceptibility of PEE cells to 
      PRRSV, we conclude that the PEE cell line generated will be useful for growth of 
      PRRSV and further studies on the underlying mechanism for PRRSV infection of PEE 
      cells. The finding of the susceptibility of PEE cells to PRRSV may provide an 
      alternative explanation for PRRSV-induced reproductive failure.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Feng, Lili
AU  - Feng L
AD  - College of Veterinary Medicine, Yangzhou University, 48 Wenhui Road, Yangzhou 
      225009, Jiangsu, PR China.
FAU - Zhang, Xinyu
AU  - Zhang X
FAU - Xia, Xiaoli
AU  - Xia X
FAU - Li, Yangyang
AU  - Li Y
FAU - He, Shan
AU  - He S
FAU - Sun, Huaichang
AU  - Sun H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121205
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Endometrium/*virology
MH  - Endothelial Cells/*virology
MH  - Female
MH  - Porcine respiratory and reproductive syndrome virus/growth & 
      development/*physiology
MH  - *Swine
MH  - Virus Replication
EDAT- 2012/12/12 06:00
MHDA- 2013/07/03 06:00
CRDT- 2012/12/11 06:00
PHST- 2012/09/21 00:00 [received]
PHST- 2012/11/23 00:00 [revised]
PHST- 2012/11/26 00:00 [accepted]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
AID - S0168-1702(12)00460-1 [pii]
AID - 10.1016/j.virusres.2012.11.015 [doi]
PST - ppublish
SO  - Virus Res. 2013 Jan;171(1):209-15. doi: 10.1016/j.virusres.2012.11.015. Epub 2012 
      Dec 5.