PMID- 23221481
OWN - NLM
STAT- MEDLINE
DCOM- 20130610
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 30
IP  - 4
DP  - 2012
TI  - Differential response of MC3T3-E1 and human mesenchymal stem cells to inositol 
      hexakisphosphate.
PG  - 974-86
LID - 10.1159/000341474 [doi]
AB  - BACKGROUND: Inositol hexakisphosphate (IP6) has been found to have an important 
      role in biomineralization. METHODS: Because the complete mechanism of action of 
      IP6 on osteoblasts is not fully understood and its potential use in the primary 
      prevention of osteoporosis, we examined the direct effect of IP6 on cell 
      viability and differentiation of MC3T3-E1 cells and on differentiation of human 
      umbilical cord mesenchymal stem cells (hUC-MSCs). RESULTS: We show that IP6 has 
      different effects depending on the origin of the cell target. Thus, while IP6 
      decreased gene expression of osteoblast markers and mineralization in MC3T3-E1 
      cells without negatively affecting cell viability and ALP activity, an increase 
      in gene expression of ALP was observed in hUC-MSCs committed to the osteoblastic 
      lineage. This increasing effect of IP6 on ALP mRNA expression levels was reversed 
      by the addition of a selective inhibitor of IP6 kinase, suggesting that the 
      effect of IP6 might be due through its pyrophosphorylated derivatives. Besides, 
      Rankl mRNA levels were decreased after IP6 treatment in MC3T3-E1 cells, pointing 
      to a paracrine effect on osteoclasts. CONCLUSION: Our results indicate that IP6 
      has different effects on osteoblast differentiation depending on the cell type 
      and origin. However, further studies are needed to examine the net effect of IP6 
      on bone formation and its potential as novel antiosteoporosis drug.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Arriero, Maria Del Mar
AU  - Arriero Mdel M
AD  - Department of Fundamental Biology and Health Sciences, Research Institute on 
      Health Sciences (IUNICS), University of Balearic Islands, Palma de Mallorca, 
      Spain.
FAU - Ramis, Joana M
AU  - Ramis JM
FAU - Perello, Joan
AU  - Perello J
FAU - Monjo, Marta
AU  - Monjo M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120920
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
RN  - 7IGF0S7R8I (Phytic Acid)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - 3T3 Cells
MH  - Alkaline Phosphatase/genetics/metabolism
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice
MH  - Osteoblasts/cytology/*metabolism
MH  - Phytic Acid/*metabolism
MH  - RANK Ligand/genetics
MH  - RNA, Messenger/genetics
EDAT- 2012/12/12 06:00
MHDA- 2013/06/12 06:00
CRDT- 2012/12/11 06:00
PHST- 2012/08/29 00:00 [accepted]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
AID - 000341474 [pii]
AID - 10.1159/000341474 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2012;30(4):974-86. doi: 10.1159/000341474. Epub 2012 Sep 
      20.