PMID- 2322285
OWN - NLM
STAT- MEDLINE
DCOM- 19900507
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 167
IP  - 3
DP  - 1990 Mar 30
TI  - Tissue inhibitor of metalloproteases (TIMP) is matrix associated in aortic 
      tissue: report of a radioimmunoassay.
PG  - 898-903
AB  - Tissue inhibitor of metalloproteinases (TIMP) is the major inhibitor of 
      collagenase, gelatinase, proteoglycanase, stromelysin, and metalloelastases. An 
      imbalance between proteases and inhibitors has been implicated in numerous 
      disease processes including tumor invasion, rheumatoid arthritis, emphysema, and 
      aortic aneurysm disease. The purpose of this investigation was to develop a 
      polyclonal antibody to recombinant TIMP and establish an immunoassay to measure 
      immunoreactive protein in normal and diseased tissues. A polyclonal antibody was 
      produced in rabbit against recombinant human TIMP which was characterized and 
      used to establish a radioimmunoassay. The assay was used to measure 
      immunoreactive protein in fibroblast conditioned medium, human serum, and aortic 
      extracts. There was more immunoreactive TIMP in matrix associated urea extracts 
      than soluble salt extracts from human aorta, suggesting that TIMP is matrix 
      associated. The sensitivity of the assay enables the specific measurement of this 
      inhibitor in serum, fibroblast culture medium, and tissue extracts.
FAU - Brophy, C M
AU  - Brophy CM
AD  - Department of Surgery, Yale University School of Medicine, New Haven, CT 06510.
FAU - Marks, W H
AU  - Marks WH
FAU - Reilly, J M
AU  - Reilly JM
FAU - Tilson, M D
AU  - Tilson MD
LA  - eng
GR  - HL07964-02/HL/NHLBI NIH HHS/United States
GR  - R0-1 29325/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Glycoproteins)
RN  - 0 (Immune Sera)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - Aorta, Abdominal/*analysis
MH  - Cells, Cultured
MH  - Fibroblasts/analysis
MH  - Glycoproteins/*analysis/blood/immunology
MH  - Humans
MH  - Immune Sera
MH  - Immunoelectrophoresis
MH  - Metalloendopeptidases/*antagonists & inhibitors
MH  - Muscle, Smooth, Vascular/*analysis
MH  - Radioimmunoassay/methods
MH  - Recombinant Proteins/analysis
MH  - Skin/analysis
MH  - Tissue Inhibitor of Metalloproteinases
EDAT- 1990/03/30 00:00
MHDA- 1990/03/30 00:01
CRDT- 1990/03/30 00:00
PHST- 1990/03/30 00:00 [pubmed]
PHST- 1990/03/30 00:01 [medline]
PHST- 1990/03/30 00:00 [entrez]
AID - 0006-291X(90)90608-P [pii]
AID - 10.1016/0006-291x(90)90608-p [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1990 Mar 30;167(3):898-903. doi: 
      10.1016/0006-291x(90)90608-p.