PMID- 23224948
OWN - NLM
STAT- MEDLINE
DCOM- 20130718
LR  - 20220318
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 169
IP  - 2
DP  - 2013 Jan
TI  - Identifying the regulative role of NF-kappaB binding sites within promoter region of 
      human matrix metalloproteinase 9 (mmp-9) by TNF-alpha induction.
PG  - 438-49
LID - 10.1007/s12010-012-9958-3 [doi]
AB  - Matrix metalloproteinase 9 (MMP-9), a member of MMP family, is involved in many 
      physiological processes, including cardiovascular disease (CVD). Tumor necrosis 
      factor-alpha (TNF-alpha) is considered a cytokine with pleiotropic biological 
      capabilities and leads to the process of CVD when TNF-alpha is abnormally released 
      and stimulates MMP-9 expression and activation. In this study, we investigated 
      the molecular mechanism of TNF-alpha-regulated MMP-9 expression. The experimental 
      results confirm that TNF-alpha could upregulate MMP-9 expression in heart myoblast 
      H9c2 cells of rat. To evaluate the MMP-9 regulation at transcriptional level, a 
      DNA fragment of 2.2 kb (-2168/+18) of human mmp-9 promoter region was cloned and 
      constructed in a vector of luciferase reporter gene. The 2.2-kb sequences were 
      identified as having three candidate nuclear factor-kappa B (NF-kappaB) binding sites: 
      NF-kappaB I (-1418/-1409), NF-kappaB II (-626/-617), and NF-kappaB III (-353/-345). A series 
      of reporter vectors with the mutated NF-kappaB sites of mmp-9 promoter sequences were 
      constructed and transfected into H9c2 cells. The results show that the NF-kappaB II 
      binding site (-626/-617) within the promoter region of mmp-9 plays a key role in 
      upregulation of mmp-9 expression by TNF-alpha induction. In addition, we also first 
      identified that the NF-kappaB I, similar to c-Rel, might be one of the NF-kappaB families 
      to regulate mmp-9 expression.
FAU - Wu, Hsi Tien
AU  - Wu HT
AD  - Department of BioAgricultural Science, National Chia Yi University, 300 Syuefu 
      Rd., Chiayi 60004, Taiwan. htwu@mail.ncyu.edu.tw
FAU - Sie, Syu Sheng
AU  - Sie SS
FAU - Kuan, Tang Ching
AU  - Kuan TC
FAU - Lin, Chih Sheng
AU  - Lin CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121206
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Base Sequence
MH  - Binding Sites
MH  - Gene Expression Regulation, Enzymologic/*genetics
MH  - Humans
MH  - Matrix Metalloproteinase 9/*genetics
MH  - Molecular Sequence Data
MH  - NF-kappa B/*genetics
MH  - Promoter Regions, Genetic/*genetics
MH  - Protein Binding
MH  - Tumor Necrosis Factor-alpha/*genetics
MH  - Up-Regulation/genetics
EDAT- 2012/12/12 06:00
MHDA- 2013/07/19 06:00
CRDT- 2012/12/11 06:00
PHST- 2012/08/01 00:00 [received]
PHST- 2012/11/06 00:00 [accepted]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/07/19 06:00 [medline]
AID - 10.1007/s12010-012-9958-3 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2013 Jan;169(2):438-49. doi: 10.1007/s12010-012-9958-3. 
      Epub 2012 Dec 6.