PMID- 23224981
OWN - NLM
STAT- MEDLINE
DCOM- 20130827
LR  - 20211021
IS  - 1573-904X (Electronic)
IS  - 0724-8741 (Print)
IS  - 0724-8741 (Linking)
VI  - 30
IP  - 4
DP  - 2013 Apr
TI  - Location-dependent coronary artery diffusive and convective mass transport 
      properties of a lipophilic drug surrogate measured using nonlinear microscopy.
PG  - 1147-60
LID - 10.1007/s11095-012-0950-y [doi]
AB  - PURPOSE: Arterial wall mass transport properties dictate local distribution of 
      biomolecules or locally delivered dugs. Knowing how these properties vary between 
      coronary artery locations could provide insight into how therapy efficacy is 
      altered between arterial locations. METHODS: We introduced an indocarbocyanine 
      drug surrogate to the lumens of left anterior descending and right coronary 
      (LADC; RC) arteries from pigs with or without a pressure gradient. Interstitial 
      fluorescent intensity was measured on live samples with multiphoton microscopy. 
      We also measured binding to porcine coronary SMCs in monoculture. RESULTS: 
      Diffusive transport constants peaked in the middle sections of the LADC and RC 
      arteries by 2.09 and 2.04 times, respectively, compared to the proximal and 
      distal segments. There was no statistical difference between the average 
      diffusivity value between LADC and RC arteries. The convection coefficients had 
      an upward trend down each artery, with the RC being higher than the LADC by 3.89 
      times. CONCLUSIONS: This study demonstrates that the convective and diffusive 
      transport of lipophilic molecules changes between the LADC and the RC arteries as 
      well as along their length. These results may have important implications in 
      optimizing drug delivery for the treatment of coronary artery disease.
FAU - Keyes, Joseph T
AU  - Keyes JT
AD  - Graduate Interdisciplinary Program in Biomedical Engineering, The University of 
      Arizona, Tucson, Arizona, 85721, USA.
FAU - Simon, Bruce R
AU  - Simon BR
FAU - Vande Geest, Jonathan P
AU  - Vande Geest JP
LA  - eng
GR  - S10 RR023737/RR/NCRR NIH HHS/United States
GR  - T32 HL007955/HL/NHLBI NIH HHS/United States
GR  - 1S10RR023737-01/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20121207
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - 0 (Carbocyanines)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Animals
MH  - Carbocyanines/administration & dosage/*pharmacokinetics
MH  - Cells, Cultured
MH  - Coronary Vessels/*metabolism
MH  - Diffusion
MH  - Fluorescent Dyes/administration & dosage/*pharmacokinetics
MH  - Image Processing, Computer-Assisted
MH  - Microscopy, Fluorescence, Multiphoton
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Pressure
MH  - Swine
PMC - PMC3818903
MID - NIHMS427352
EDAT- 2012/12/12 06:00
MHDA- 2013/08/28 06:00
PMCR- 2014/04/01
CRDT- 2012/12/11 06:00
PHST- 2012/09/19 00:00 [received]
PHST- 2012/11/27 00:00 [accepted]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/08/28 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - 10.1007/s11095-012-0950-y [doi]
PST - ppublish
SO  - Pharm Res. 2013 Apr;30(4):1147-60. doi: 10.1007/s11095-012-0950-y. Epub 2012 Dec 
      7.