PMID- 23225804
OWN - NLM
STAT- MEDLINE
DCOM- 20140114
LR  - 20211021
IS  - 1976-2437 (Electronic)
IS  - 0513-5796 (Print)
IS  - 0513-5796 (Linking)
VI  - 54
IP  - 1
DP  - 2013 Jan 1
TI  - Glioma stem cell-targeted dendritic cells as a tumor vaccine against malignant 
      glioma.
PG  - 92-100
LID - 10.3349/ymj.2013.54.1.92 [doi]
AB  - PURPOSE: Cancer stem cells have recently been thought to be closely related to 
      tumor development and reoccurrence. It may be a promising way to cure malignant 
      glioma by using glioma stem cell-targeted dendritic cells as a tumor vaccine. In 
      this study, we explored whether pulsing dendritic cells with antigens of glioma 
      stem cells was a potent way to induce specific cytotoxic T lymphocytes and 
      anti-tumor immunity. MATERIALS AND METHODS: Cancer stem cells were cultured from 
      glioma cell line U251. Lysate of glioma stem cells was obtained by the repeated 
      freezing and thawing method. Dendritic cells (DCs) were induced and cultured from 
      the murine bone marrow cells, the biological characteristics were detected by 
      electron microscope and flow cytometry. The DC vaccine was obtained by mixing DCs 
      with lysate of glioma stem cells. The DC vaccine was charactirizated through the 
      mixed lymphocyte responses and cell killing experiment in vitro. Level of 
      interferon-gamma (IFN-gamma) in the supernatant was checked by ELISA. RESULTS: After 
      stimulation of lysate of glioma stem cell, expression of surface molecules of DC 
      was up-regulated, including CD80, CD86, CD11C and MHC-II. DCs pulsed with lysate 
      of glioma stem cells were more effective than the control group in stimulating 
      original glioma cells-specific cytotoxic T lymphocytes responses, killing glioma 
      cells and boosting the secretion of IFN-gamma in vitro. CONCLUSION: The results 
      demonstrated DCs loaded with antigens derived from glioma stem cells can 
      effectively stimulate naive T cells to form specific cytotoxic T cells, kill 
      glioma cells cultured in vitro.
FAU - Ji, Baowei
AU  - Ji B
AD  - Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuchang 
      District, Wuhan, China.
FAU - Chen, Qianxue
AU  - Chen Q
FAU - Liu, Baohui
AU  - Liu B
FAU - Wu, Liquan
AU  - Wu L
FAU - Tian, Daofeng
AU  - Tian D
FAU - Guo, Zhentao
AU  - Guo Z
FAU - Yi, Wei
AU  - Yi W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - Yonsei Med J
JT  - Yonsei medical journal
JID - 0414003
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cancer Vaccines)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/immunology
MH  - Apoptosis
MH  - Brain Neoplasms/*therapy
MH  - Cancer Vaccines/*therapeutic use
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Dendritic Cells/*cytology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Glioma/*therapy
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neoplasm Transplantation
MH  - Neoplastic Stem Cells/*cytology
MH  - T-Lymphocytes, Cytotoxic/immunology
PMC - PMC3521251
COIS- The authors have no financial conflicts of interest.
EDAT- 2012/12/12 06:00
MHDA- 2014/01/15 06:00
PMCR- 2013/01/01
CRDT- 2012/12/11 06:00
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2014/01/15 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 201301092 [pii]
AID - 10.3349/ymj.2013.54.1.92 [doi]
PST - ppublish
SO  - Yonsei Med J. 2013 Jan 1;54(1):92-100. doi: 10.3349/ymj.2013.54.1.92.