PMID- 23226943
OWN - NLM
STAT- MEDLINE
DCOM- 20130411
LR  - 20211021
IS  - 1110-7251 (Electronic)
IS  - 1110-7243 (Print)
IS  - 1110-7243 (Linking)
VI  - 2012
DP  - 2012
TI  - Genistein attenuates vascular endothelial impairment in ovariectomized 
      hyperhomocysteinemic rats.
PG  - 730462
LID - 10.1155/2012/730462 [doi]
LID - 730462
AB  - Hyperhomocysteinemia (HHcy) is a well-known independent risk factor for vascular 
      diseases in the general population. This study was to explore the effect of 
      genistein (GST), a natural bioactive compound derived from legumes, on 
      HHcy-induced vascular endothelial impairment in ovariectomized rats in vivo. 
      Thirty-two adult female Wistar rats were assigned randomly into four groups (n = 
      8): (a) Con: control; (b) Met: 2.5% methionine diet; (c) OVX + Met: ovariectomy + 
      2.5% methionine diet; (d) OVX + Met + GST: ovariectomy + 2.5% methionine diet + 
      supplementation with genistein. After 12 wk of different treatment, the rats' 
      blood, toracic aortas and liver samples were collected for analysis. Results 
      showed that high-methionine diet induced both elevation of plasma Hcy and 
      endothelial dysfunction, and ovariectomy deteriorated these injuries. Significant 
      improvement of both functional and morphological changes of vascular endothelium 
      was observed in OVX + Met + GST group; meanwhile the plasma Hcy levels decreased 
      remarkably. There were significant elevations of plasma ET-1 and liver MDA levels 
      in ovariectomized HHcy rats, and supplementation with genistein could attenuate 
      these changes. These results implied that genistein could lower the elevated Hcy 
      levels, and prevent the development of endothelial impairment in ovariectomized 
      HHcy rats. This finding may shed a novel light on the anti-atherogenic activities 
      of genistein in HHcy patients.
FAU - Zhen, Panpan
AU  - Zhen P
AD  - Department of Pathology, The Luhe Teaching Hospital of the Capital Medical 
      University, 82 Xinhuanan Road, Tongzhou District, Beijing 101149, China.
FAU - Zhao, Qian
AU  - Zhao Q
FAU - Hou, Dandan
AU  - Hou D
FAU - Liu, Teng
AU  - Liu T
FAU - Jiang, Dongqiao
AU  - Jiang D
FAU - Duan, Jinhong
AU  - Duan J
FAU - Lu, Lingqiao
AU  - Lu L
FAU - Wang, Wen
AU  - Wang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121106
PL  - United States
TA  - J Biomed Biotechnol
JT  - Journal of biomedicine & biotechnology
JID - 101135740
RN  - 0 (Endothelin-1)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 169D1260KM (Nitroprusside)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 4TI98Z838E (Estradiol)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - DH2M523P0H (Genistein)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Aorta, Thoracic/drug effects/pathology/physiopathology/ultrastructure
MH  - Endothelin-1/blood
MH  - Endothelium, Vascular/drug effects/*pathology/*physiopathology/ultrastructure
MH  - Estradiol/blood
MH  - Female
MH  - Genistein/*pharmacology
MH  - Homocysteine/blood
MH  - Hyperhomocysteinemia/blood/*pathology/*physiopathology
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Liver/drug effects/metabolism
MH  - Malondialdehyde/metabolism
MH  - Muscle Contraction/drug effects
MH  - Nitroprusside/pharmacology
MH  - *Ovariectomy
MH  - Phenylephrine/pharmacology
MH  - Rats
MH  - Rats, Wistar
PMC - PMC3511852
EDAT- 2012/12/12 06:00
MHDA- 2013/04/12 06:00
PMCR- 2012/11/06
CRDT- 2012/12/11 06:00
PHST- 2012/03/27 00:00 [received]
PHST- 2012/10/14 00:00 [revised]
PHST- 2012/10/15 00:00 [accepted]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/04/12 06:00 [medline]
PHST- 2012/11/06 00:00 [pmc-release]
AID - 10.1155/2012/730462 [doi]
PST - ppublish
SO  - J Biomed Biotechnol. 2012;2012:730462. doi: 10.1155/2012/730462. Epub 2012 Nov 6.