PMID- 23227154
OWN - NLM
STAT- MEDLINE
DCOM- 20130611
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 12
DP  - 2012
TI  - Methamphetamine administration targets multiple immune subsets and induces 
      phenotypic alterations suggestive of immunosuppression.
PG  - e49897
LID - 10.1371/journal.pone.0049897 [doi]
LID - e49897
AB  - Methamphetamine (Meth) is a widely abused stimulant and its users are at 
      increased risk for multiple infectious diseases. To determine the impact of meth 
      on the immune system, we utilized a murine model that simulates the process of 
      meth consumption in a typical addict. Our phenotypic analysis of leukocytes from 
      this dose escalation model revealed that meth affected key immune subsets. Meth 
      administration led to a decrease in abundance of natural killer (NK) cells and 
      the remaining NK cells possessed a phenotype suggesting reduced responsiveness. 
      Dendritic cells (DCs) and Gr-1(high) monocytes/macrophages were also decreased in 
      abundance while Gr-1(low) monocytes/macrophages appear to show signs of 
      perturbation. CD4 and CD8 T cell subsets were affected by methamphetamine, both 
      showing a reduction in antigen-experienced subsets. CD4 T cells also exhibited 
      signs of activation, with increased expression of CD150 on CD226-expressing cells 
      and an expansion of KLRG1(+), FoxP3(-) cells. These results exhibit that meth has 
      the ability to disrupt immune homeostasis and impact key subsets of leukocytes 
      which may leave users more vulnerable to pathogens.
FAU - Harms, Robert
AU  - Harms R
AD  - Department of Surgery-Transplant, University of Nebraska Medical Center, Omaha, 
      Nebraska, United States of America. rharms@unmc.edu
FAU - Morsey, Brenda
AU  - Morsey B
FAU - Boyer, Craig W
AU  - Boyer CW
FAU - Fox, Howard S
AU  - Fox HS
FAU - Sarvetnick, Nora
AU  - Sarvetnick N
LA  - eng
GR  - R01 DA032513/DA/NIDA NIH HHS/United States
GR  - NIH RO1:345160-2058101/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20121205
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 44RAL3456C (Methamphetamine)
SB  - IM
MH  - Animals
MH  - Disease Susceptibility/*immunology
MH  - Flow Cytometry
MH  - Immunocompromised Host
MH  - Immunophenotyping
MH  - Lymphocytes/*drug effects/immunology
MH  - Macrophages/*drug effects/immunology
MH  - Male
MH  - Methamphetamine/*administration & dosage/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Models, Animal
MH  - Monocytes/*drug effects/immunology
PMC - PMC3515581
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/12/12 06:00
MHDA- 2013/06/12 06:00
PMCR- 2012/12/05
CRDT- 2012/12/11 06:00
PHST- 2012/07/23 00:00 [received]
PHST- 2012/10/17 00:00 [accepted]
PHST- 2012/12/11 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
PHST- 2012/12/05 00:00 [pmc-release]
AID - PONE-D-12-22183 [pii]
AID - 10.1371/journal.pone.0049897 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(12):e49897. doi: 10.1371/journal.pone.0049897. Epub 2012 Dec 5.