PMID- 23228673
OWN - NLM
STAT- MEDLINE
DCOM- 20130925
LR  - 20130319
IS  - 1872-8111 (Electronic)
IS  - 0168-0102 (Linking)
VI  - 75
IP  - 2
DP  - 2013 Feb
TI  - Neural crest-derived horizontal basal cells as tissue stem cells in the adult 
      olfactory epithelium.
PG  - 112-20
LID - S0168-0102(12)00231-3 [pii]
LID - 10.1016/j.neures.2012.11.005 [doi]
AB  - Horizontal basal cells (HBCs) have garnered attention as tissue stem cells of the 
      olfactory epithelium (OE); however, these cells' exact lineage and their 
      contributions to OE regeneration remain unknown. Neural crest-derived cells 
      (NCDCs) have been shown to possess stem cell properties and to participate in the 
      normal development of the OE. However, the contributions of NCDCs to both normal 
      and regenerating adult OE remain unclear. In this study, we investigated the 
      contribution of NCDCs to the OE, focusing particularly on HBCs. Using 
      immunohistochemistry, we observed the OE of P0-Cre/EGFP mice expressing 
      EGFP-tagged NCDCs at several stages of normal development along with regenerated 
      OE following methimazole treatment. We observed EGFP expression in the HBCs, 
      sustentacular cells (SUSs), Bowman's glands, olfactory receptor neurons (ORNs), 
      and olfactory ensheathing cells of 6-week-old mice. No ectopic Cre expression was 
      identified. Although HBCs at late embryonic stages were placode-derived (i.e., 
      EGFP-negative), we found that EGFP+ HBCs alternatively increased with the 
      decrease of placode-derived HBCs during maturation. In regenerated OE, the 
      percentages of neural crest-derived ORNs and SUSs significantly increased 
      compared with normal OE. These results suggest that NCDCs contribute greatly to 
      the adult HBC population and that they are important for the maintenance of the 
      OE.
CI  - Copyright (c) 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society. All 
      rights reserved.
FAU - Suzuki, Jun
AU  - Suzuki J
AD  - Division of Developmental Neuroscience, United Centers for Advanced Research and 
      Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1, 
      Seiryo-Machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Yoshizaki, Kaichi
AU  - Yoshizaki K
FAU - Kobayashi, Toshimitsu
AU  - Kobayashi T
FAU - Osumi, Noriko
AU  - Osumi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121208
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
SB  - IM
MH  - Aging
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Neural Crest/*cytology/metabolism
MH  - Neural Stem Cells/*cytology/metabolism
MH  - Olfactory Mucosa/*cytology/metabolism
MH  - Olfactory Receptor Neurons/cytology/*metabolism
EDAT- 2012/12/12 06:00
MHDA- 2013/09/26 06:00
CRDT- 2012/12/12 06:00
PHST- 2012/07/22 00:00 [received]
PHST- 2012/10/26 00:00 [revised]
PHST- 2012/11/07 00:00 [accepted]
PHST- 2012/12/12 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/09/26 06:00 [medline]
AID - S0168-0102(12)00231-3 [pii]
AID - 10.1016/j.neures.2012.11.005 [doi]
PST - ppublish
SO  - Neurosci Res. 2013 Feb;75(2):112-20. doi: 10.1016/j.neures.2012.11.005. Epub 2012 
      Dec 8.