PMID- 23229554
OWN - NLM
STAT- MEDLINE
DCOM- 20130326
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 288
IP  - 3
DP  - 2013 Jan 18
TI  - Hypoxia-inducible Factor-1 (HIF-1)-independent hypoxia response of the small heat 
      shock protein hsp-16.1 gene regulated by chromatin-remodeling factors in the 
      nematode Caenorhabditis elegans.
PG  - 1582-9
LID - 10.1074/jbc.M112.401554 [doi]
AB  - Oxygen deprivation is accompanied by the coordinated expression of numerous 
      hypoxia-responsive genes, many of which are controlled by hypoxia-inducible 
      factor-1 (HIF-1). However, the cellular response to hypoxia is not likely to be 
      mediated by HIF-1 alone, and little is known about HIF-1-independent hypoxia 
      responses. To better establish the molecular mechanisms of HIF-1-independent 
      hypoxia responses, we sought to characterize the molecular basis of the hypoxia 
      response of the hsp-16.1 gene in the nematode Caenorhabditis elegans; this gene 
      has been shown to be induced by hypoxia independently of hif-1. Using affinity 
      purification followed by LC-MS/MS, we identified HMG-1.2 as a protein that binds 
      to a specific promoter region under hypoxic conditions. By systematic prediction 
      followed by validation of these interactions through RNAi, we identified the 
      chromatin modifiers isw-1 and hda-1, histone H4, and NURF-1 chromatin-remodeling 
      factors as new components of the hif-1-independent hypoxia response. These data 
      suggest that the modulation of nucleosome positioning at the hsp-16.1 promoter 
      may be important for the hypoxia response. In addition, we found that calcineurin 
      acts independently of hif-1 to modulate the cellular response to hypoxia and that 
      calcium ions are necessary for the induction of hsp-16.1 under hypoxic 
      conditions.
FAU - Lee, Jihyun
AU  - Lee J
AD  - Institute of Molecular Biology and Genetics, Research Center for Cellulomics, 
      Department of Biological Sciences, World Class University Department of 
      Biophysics and Chemical Biology, Seoul National University, Seoul 151-742, South 
      Korea.
FAU - Lee, Junho
AU  - Lee J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121210
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (Histones)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (NURF-1 protein, C elegans)
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.5.1.- (HDA-1 protein, C elegans)
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/*genetics/metabolism
MH  - Caenorhabditis elegans Proteins/genetics/metabolism
MH  - Calcium/metabolism
MH  - Chromatin Assembly and Disassembly
MH  - Chromatography, Liquid
MH  - Chromosomal Proteins, Non-Histone/genetics/metabolism
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - *Gene Expression Regulation
MH  - Gene Silencing
MH  - Heat-Shock Proteins/*genetics/metabolism
MH  - High Mobility Group Proteins/*genetics/metabolism
MH  - Histone Deacetylases/genetics/metabolism
MH  - Histones/genetics/metabolism
MH  - Humans
MH  - Hypoxia/*genetics/metabolism
MH  - Hypoxia-Inducible Factor 1/genetics/metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction
MH  - Tandem Mass Spectrometry
PMC - PMC3548468
EDAT- 2012/12/12 06:00
MHDA- 2013/03/27 06:00
PMCR- 2014/01/18
CRDT- 2012/12/12 06:00
PHST- 2012/12/12 06:00 [entrez]
PHST- 2012/12/12 06:00 [pubmed]
PHST- 2013/03/27 06:00 [medline]
PHST- 2014/01/18 00:00 [pmc-release]
AID - S0021-9258(20)46572-X [pii]
AID - M112.401554 [pii]
AID - 10.1074/jbc.M112.401554 [doi]
PST - ppublish
SO  - J Biol Chem. 2013 Jan 18;288(3):1582-9. doi: 10.1074/jbc.M112.401554. Epub 2012 
      Dec 10.