PMID- 23231431
OWN - NLM
STAT- MEDLINE
DCOM- 20130801
LR  - 20181202
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 14
IP  - 2
DP  - 2013 Feb 11
TI  - Control over the gradient differentiation of rat BMSCs on a PCL membrane with 
      surface-immobilized alendronate gradient.
PG  - 342-9
LID - 10.1021/bm301523p [doi]
AB  - Gradient biomaterials can offer progressively changing signals to specific tissue 
      interface, and thereby modulate the conjunction between different tissues. A 
      linear density gradient of alendronate (Aln), a molecule that is capable of 
      promoting osteogenic differentiation of bone mesenchymal stem cells (BMSCs), was 
      created on an aminolyzed poly(epsilon-caprolactone) (PCL) membrane. X-ray photoelectron 
      spectroscopy and quartz crystal microbalance with dissipation revealed the linear 
      increase of the Aln amount as a function of the position on the PCL membrane. By 
      contrast, the surface wettability and energy were kept unchanged. The 
      surface-grafted Aln showed a stronger ability to induce the osteogenic 
      differentiation of rat BMSCs than its counterpart in culture medium of the same 
      amount, and the osteo-inductive culture medium. On the Aln-grafted gradient 
      surface, the BMSCs showed gradient osteogenic differentiation as a function of 
      membrane position in terms of cell morphology, alkaline phosphatase activity, 
      calcium deposition, and the expression of osteogenesis marker proteins including 
      collagen type I (COL I), Runt-related transcription factor 2 (Runx2), and 
      osteocalcin (OCN).
FAU - Zhu, Yang
AU  - Zhu Y
AD  - MOE of Key Laboratory of Macromolecular Synthesis and Functionalization, 
      Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 
      China.
FAU - Mao, Zhengwei
AU  - Mao Z
FAU - Gao, Changyou
AU  - Gao C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130114
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Biocompatible Materials)
RN  - 0 (Collagen Type I)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Polyesters)
RN  - 0 (Runx2 protein, rat)
RN  - 104982-03-8 (Osteocalcin)
RN  - 24980-41-4 (polycaprolactone)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
RN  - X1J18R4W8P (Alendronate)
SB  - IM
MH  - Alendronate/chemistry/metabolism/*pharmacology
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Biocompatible Materials
MH  - Bone Marrow Cells/cytology/metabolism
MH  - Calcium/chemistry
MH  - Cell Adhesion
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Collagen Type I/biosynthesis
MH  - Core Binding Factor Alpha 1 Subunit/biosynthesis
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Osteocalcin/biosynthesis
MH  - Osteogenesis/*drug effects
MH  - Photoelectron Spectroscopy
MH  - Polyesters/chemistry
MH  - Rats
EDAT- 2012/12/13 06:00
MHDA- 2013/08/02 06:00
CRDT- 2012/12/13 06:00
PHST- 2012/12/13 06:00 [entrez]
PHST- 2012/12/13 06:00 [pubmed]
PHST- 2013/08/02 06:00 [medline]
AID - 10.1021/bm301523p [doi]
PST - ppublish
SO  - Biomacromolecules. 2013 Feb 11;14(2):342-9. doi: 10.1021/bm301523p. Epub 2013 Jan 
      14.