PMID- 23232037
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130114
LR  - 20211021
IS  - 2049-1891 (Electronic)
IS  - 1674-9782 (Print)
IS  - 1674-9782 (Linking)
VI  - 3
IP  - 1
DP  - 2012 Dec 11
TI  - Changes of hepatic biochemical parameters and proteomics in broilers with 
      cold-induced ascites.
PG  - 41
LID - 10.1186/2049-1891-3-41 [doi]
AB  - Ascites syndrome is still a problem for chicken industry in various parts of the 
      world. Despite the intensive investigations of this syndrome for many years, its 
      pathogenesis remains unclear. The objective of this study was to analyze the 
      difference in hepatic proteomics between ascites and healthy broilers by 
      two-dimensional electrophoresis (2-DE) and matrix-assisted laser 
      desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Changes of 
      biochemical parameters of liver and blood were also determined. The results 
      indicated that red blood cell counts (RBC), hematocrit (HCT) and haemoglobin 
      (HGB) of ascites broilers were significantly greater than healthy broilers. 
      Hepatic malondialdehyde (MDA) level of ascites broilers was significantly 
      increased, and the activity of total superoxide dismutase (T-SOD) was 
      significantly decreased. Hepatic lactic acid (LD) level of ascitic broilers were 
      significantly lower than healthy ones. Serum glucose and cholesterol level of 
      ascites broilers were significantly increased, and serum globulin level was 
      significantly decreased in ascites broilers. There was no significant difference 
      in triglyceride (TG) and blood urea nitrogen (BUN) level. The activity of liver 
      hexokinase (HK) and succinodehydrogenase (SDH) in ascites broilers was 
      significantly decreased, and there was no significant difference in the activity 
      of liver pyruvate kinase (PK) and Na+-K+-ATPase. The hepatic proteomics analysis 
      showed that 18 proteins expression difference were identified between ascites and 
      healthy broilers. These proteins were mainly involved in: 1) cytoskeleton; 2) 
      glucose, lipids and amino acid metabolism; 3) cell secretion; 4) cell apoptosis; 
      5) signal transduction; 6) immune and inflammatory response; and 7) cellular 
      redox homeostasis. Mitochondrial isoform phosphoenolpyruvate carboxykinase 
      (M-PEPCK) mainly participates in gluconeogenesis of chicken liver. In conclusion, 
      liver oxidative damage was significantly aggravated, but antioxidant capacity was 
      decreased in cold-induced ascites broilers. Serum glucose level was significantly 
      increased, with liver M-PEPCK expression higher in ascites broilers, which 
      implied that some potential regulatory reagents may reduce ascites susceptibility 
      and mortality under cold temperature by increasing liver gluconeogenesis level.
FAU - Wang, Yongwei
AU  - Wang Y
AD  - State Key Lab of Animal Nutrition, College of Animal Science and Technology, 
      China Agricultural University, Beijing 100193, PR China. guoyum@cau.edu.cn.
FAU - Guo, Yuming
AU  - Guo Y
FAU - Ning, Dong
AU  - Ning D
FAU - Peng, Yunzhi
AU  - Peng Y
FAU - Cai, Hong
AU  - Cai H
FAU - Tan, Jianzhuang
AU  - Tan J
FAU - Yang, Ying
AU  - Yang Y
FAU - Liu, Dan
AU  - Liu D
LA  - eng
PT  - Journal Article
DEP - 20121211
PL  - England
TA  - J Anim Sci Biotechnol
JT  - Journal of animal science and biotechnology
JID - 101581293
PMC - PMC3542246
EDAT- 2012/12/13 06:00
MHDA- 2012/12/13 06:01
PMCR- 2012/12/11
CRDT- 2012/12/13 06:00
PHST- 2012/09/14 00:00 [received]
PHST- 2012/12/03 00:00 [accepted]
PHST- 2012/12/13 06:00 [entrez]
PHST- 2012/12/13 06:00 [pubmed]
PHST- 2012/12/13 06:01 [medline]
PHST- 2012/12/11 00:00 [pmc-release]
AID - 2049-1891-3-41 [pii]
AID - 10.1186/2049-1891-3-41 [doi]
PST - epublish
SO  - J Anim Sci Biotechnol. 2012 Dec 11;3(1):41. doi: 10.1186/2049-1891-3-41.