PMID- 23233217
OWN - NLM
STAT- MEDLINE
DCOM- 20130517
LR  - 20121212
IS  - 1869-1889 (Electronic)
IS  - 1674-7305 (Linking)
VI  - 55
IP  - 12
DP  - 2012 Dec
TI  - Conservation and characterization of unique porcine interstitial telomeric 
      sequences.
PG  - 1029-37
LID - 10.1007/s11427-012-4420-x [doi]
AB  - Telomeres are composed of TTAGGG repeats and located at the ends of chromosomes. 
      Telomeres protect chromosomes from instability in mammals, including mice and 
      humans. Repetitive TTAGGG sequences are also found at intrachromosomal sites, 
      where they are named as interstitial telomeric sequences (ITSs). Aberrant ITSs 
      are implicated in chromosomal instability and found in cancer cells. 
      Interestingly, in pigs, vertebrate telomere sequences TTAGGG (vITSs) are also 
      localized at the centromeric region of chromosome 6, in addition to the end of 
      all chromosomes. Surprisingly, we found that botanic telomere sequences, TTTAGGG 
      (bITSs), also localize with vITSs at the centromeric regions of pig chromosome 6 
      using telomere fluorescence in situ hybridization (FISH) and by comparisons 
      between several species. Furthermore, the average lengths of vITSs are highly 
      correlated with those of the terminal telomeres (TTS). Also, pig ITSs show a high 
      incidence of telomere doublets, suggesting that pig ITSs might be unstable and 
      dynamic. Together, our results show that pig cells maintain the conserved 
      telomere sequences that are found at the ITSs from of plants and other 
      vertebrates. Further understanding of the function and regulation of pig ITSs may 
      provide new clues for evolution and chromosomal instability.
FAU - Ji, Guangzhen
AU  - Ji G
AD  - State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, 
      Nankai University, Tianjin 300071, China.
FAU - Liu, Kai
AU  - Liu K
FAU - Chen, Chengbin
AU  - Chen C
FAU - Ruan, Weimin
AU  - Ruan W
FAU - Glytsou, Christina
AU  - Glytsou C
FAU - Yang, Yang
AU  - Yang Y
FAU - Okuka, Maja
AU  - Okuka M
FAU - Song, Wenqing
AU  - Song W
FAU - Gagos, Sarantis
AU  - Gagos S
FAU - Li, Ning
AU  - Li N
FAU - Liu, Lin
AU  - Liu L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121212
PL  - China
TA  - Sci China Life Sci
JT  - Science China. Life sciences
JID - 101529880
SB  - IM
MH  - Animals
MH  - In Situ Hybridization, Fluorescence
MH  - Sister Chromatid Exchange
MH  - Swine/*genetics
MH  - *Telomere
EDAT- 2012/12/13 06:00
MHDA- 2013/05/18 06:00
CRDT- 2012/12/13 06:00
PHST- 2012/09/08 00:00 [received]
PHST- 2012/11/02 00:00 [accepted]
PHST- 2012/12/13 06:00 [entrez]
PHST- 2012/12/13 06:00 [pubmed]
PHST- 2013/05/18 06:00 [medline]
AID - 10.1007/s11427-012-4420-x [doi]
PST - ppublish
SO  - Sci China Life Sci. 2012 Dec;55(12):1029-37. doi: 10.1007/s11427-012-4420-x. Epub 
      2012 Dec 12.