PMID- 23233222
OWN - NLM
STAT- MEDLINE
DCOM- 20130517
LR  - 20131121
IS  - 1869-1889 (Electronic)
IS  - 1674-7305 (Linking)
VI  - 55
IP  - 12
DP  - 2012 Dec
TI  - Zinc reverses glycine-dependent inactivation of NMDARs in cultured rat 
      hippocampal neurons.
PG  - 1075-81
LID - 10.1007/s11427-012-4421-9 [doi]
AB  - In the presence of glutamate and co-agonists, e.g., glycine, the 
      N-methyl-D-aspartate receptor (NMDAR) plays an important role in physiological 
      and pathophysiological brain processes. Previous studies indicate glycine could 
      inhibit NMDAR responses induced by high concentration of NMDA in hippocampal 
      neurons. The mechanism underlying this inhibitory impact, however, has been 
      unclear. In this study, the whole-cell patch-clamp recording and Ca(2+) imaging 
      with Fluo-3/AM under laser scanning confocal microscope were used to analyze the 
      possible involvement of NMDAR subunits in this effect. We found that the peak 
      current of NMDARs and Ca(2+) influx induced by high concentration of NMDA were 
      reduced by treatment of glycine (0.03-10 mumol L(-1)) in a dose-dependent manner, 
      and that the glycine-dependent inhibition of NMDAR responses, which were induced 
      at 300 mumol L(-1) NMDA, was reversed by ZnCl(2) through the blocking of the NR2A 
      subunit of NMDARs, but was less influenced by ifenprodil, a NR2B inhibitor. Our 
      results suggest that the glycine-dependent inactivation of NMDARs is potentially 
      modulated by the regulatory subunit NR2A.
FAU - Li, Xia
AU  - Li X
AD  - Institute of Nautical Medicine, Nantong University, Nantong 226001, China.
FAU - Chen, Zhaoqin
AU  - Chen Z
FAU - Jiang, Zhenglin
AU  - Jiang Z
FAU - Li, Yefei
AU  - Li Y
FAU - Zhang, Yunfeng
AU  - Zhang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121212
PL  - China
TA  - Sci China Life Sci
JT  - Science China. Life sciences
JID - 101529880
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - J41CSQ7QDS (Zinc)
RN  - SY7Q814VUP (Calcium)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Glycine/*pharmacology
MH  - Hippocampus/cytology/*drug effects/metabolism
MH  - Neurons/*drug effects/metabolism
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*antagonists & inhibitors
MH  - Zinc/*pharmacology
EDAT- 2012/12/13 06:00
MHDA- 2013/05/18 06:00
CRDT- 2012/12/13 06:00
PHST- 2012/11/16 00:00 [received]
PHST- 2012/11/26 00:00 [accepted]
PHST- 2012/12/13 06:00 [entrez]
PHST- 2012/12/13 06:00 [pubmed]
PHST- 2013/05/18 06:00 [medline]
AID - 10.1007/s11427-012-4421-9 [doi]
PST - ppublish
SO  - Sci China Life Sci. 2012 Dec;55(12):1075-81. doi: 10.1007/s11427-012-4421-9. Epub 
      2012 Dec 12.