PMID- 23236188
OWN - NLM
STAT- MEDLINE
DCOM- 20130221
LR  - 20240104
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 109
IP  - 52
DP  - 2012 Dec 26
TI  - Versatile design and synthesis platform for visualizing genomes with Oligopaint 
      FISH probes.
PG  - 21301-6
LID - 10.1073/pnas.1213818110 [doi]
AB  - A host of observations demonstrating the relationship between nuclear 
      architecture and processes such as gene expression have led to a number of new 
      technologies for interrogating chromosome positioning. Whereas some of these 
      technologies reconstruct intermolecular interactions, others have enhanced our 
      ability to visualize chromosomes in situ. Here, we describe an oligonucleotide- 
      and PCR-based strategy for fluorescence in situ hybridization (FISH) and a 
      bioinformatic platform that enables this technology to be extended to any 
      organism whose genome has been sequenced. The oligonucleotide probes are 
      renewable, highly efficient, and able to robustly label chromosomes in cell 
      culture, fixed tissues, and metaphase spreads. Our method gives researchers 
      precise control over the sequences they target and allows for single and 
      multicolor imaging of regions ranging from tens of kilobases to megabases with 
      the same basic protocol. We anticipate this technology will lead to an enhanced 
      ability to visualize interphase and metaphase chromosomes.
FAU - Beliveau, Brian J
AU  - Beliveau BJ
AD  - Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
FAU - Joyce, Eric F
AU  - Joyce EF
FAU - Apostolopoulos, Nicholas
AU  - Apostolopoulos N
FAU - Yilmaz, Feyza
AU  - Yilmaz F
FAU - Fonseka, Chamith Y
AU  - Fonseka CY
FAU - McCole, Ruth B
AU  - McCole RB
FAU - Chang, Yiming
AU  - Chang Y
FAU - Li, Jin Billy
AU  - Li JB
FAU - Senaratne, Tharanga Niroshini
AU  - Senaratne TN
FAU - Williams, Benjamin R
AU  - Williams BR
FAU - Rouillard, Jean-Marie
AU  - Rouillard JM
FAU - Wu, Chao-ting
AU  - Wu CT
LA  - eng
GR  - S10 RR028832/RR/NCRR NIH HHS/United States
GR  - R42 GM097003/GM/NIGMS NIH HHS/United States
GR  - R43 GM093579/GM/NIGMS NIH HHS/United States
GR  - 1R01GM085169/GM/NIGMS NIH HHS/United States
GR  - 1S10RR028832-01/RR/NCRR NIH HHS/United States
GR  - DP1 GM106412/GM/NIGMS NIH HHS/United States
GR  - 1R43GM093579/GM/NIGMS NIH HHS/United States
GR  - R01 GM085169/GM/NIGMS NIH HHS/United States
GR  - F32 CA157188/CA/NCI NIH HHS/United States
GR  - 5R42GM097003/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121211
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/genetics
MH  - Cell Nucleus/metabolism
MH  - Chromosome Painting/*methods
MH  - Chromosomes/genetics
MH  - Drosophila melanogaster/cytology/genetics
MH  - Female
MH  - Gene Library
MH  - Genome/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Metaphase/genetics
MH  - Mice
MH  - Oligonucleotide Probes/*metabolism
MH  - Ovary/cytology/metabolism
MH  - Staining and Labeling
PMC - PMC3535588
COIS- The authors declare no conflict of interest.
EDAT- 2012/12/14 06:00
MHDA- 2013/02/22 06:00
PMCR- 2012/12/11
CRDT- 2012/12/14 06:00
PHST- 2012/12/14 06:00 [entrez]
PHST- 2012/12/14 06:00 [pubmed]
PHST- 2013/02/22 06:00 [medline]
PHST- 2012/12/11 00:00 [pmc-release]
AID - 1213818110 [pii]
AID - 201213818 [pii]
AID - 10.1073/pnas.1213818110 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21301-6. doi: 
      10.1073/pnas.1213818110. Epub 2012 Dec 11.