PMID- 23237086
OWN - NLM
STAT- MEDLINE
DCOM- 20130319
LR  - 20191112
IS  - 0001-723X (Print)
IS  - 0001-723X (Linking)
VI  - 56
IP  - 4
DP  - 2012
TI  - Complete genome sequencing of two causative viruses of cassava mosaic disease in 
      Ghana.
PG  - 305-14
AB  - Cassava mosaic disease (CMV), caused by one or a combination of cassava mosaic 
      geminiviruses, is ranked among the most important constraints to profitable and 
      efficient production of cassava. Effective control measures require in-depth 
      knowledge of the viral causative agent. Using rolling-circle amplification and 
      unique enzymes, the full genome of two species of cassava mosaic geminivirus 
      isolated from infected cassava plants in Ghana were cloned into pCambia 1300 and 
      pET-28b. The sequences of the genome were determined on an ABI sequencer and a 
      pairwise comparison was performed with other cassava-infecting geminiviruses from 
      different countries. It was revealed that cassava grown in Ghana is attacked by 
      two species of geminivirus in either single or mixed infections. These are the 
      African cassava mosaic virus (ACMV) and the East African cassava mosaic virus 
      (EACMV)-like, with high sequence similarity of 94% and 80%, respectively, between 
      the DNA-A and DNA-B components of each virus, and 66% and 41% similarity of the 
      common region (CR) (for A and B accordingly). The DNA-A of ACMV and EACMV-like 
      contained 2781 and 2800 nucleotides, respectively, while their DNA-B components 
      had 2725 and 2734 nucleotides, respectively. ACMV DNA-A was over 97% similar to 
      those of other ACMVs from the continent. In contrast, EACMV-like DNA-A was over 
      98% similar to the isolates from Cameroon and other West African countries, and 
      less than 88% similar to other EACMV species. Thus ACMV and EACMV-like were named 
      African cassava mosaic virus-Ghana and East African cassava mosaic Cameroon 
      virus-Ghana. Computer analysis revealed that their genome arrangement follows the 
      typical old world bipartite begomovirus genome. The association of these two 
      species and their interaction might account for the severe symptoms observed on 
      infected plants in the field and in the greenhouse.
FAU - Oteng-Frimpong, R
AU  - Oteng-Frimpong R
AD  - College of Agriculture and Consumer Sciences, University of Ghana, Legon, Ghana.
FAU - Levy, Y
AU  - Levy Y
FAU - Torkpo, S K
AU  - Torkpo SK
FAU - Danquah, E Y
AU  - Danquah EY
FAU - Offei, S K
AU  - Offei SK
FAU - Gafni, Y
AU  - Gafni Y
LA  - eng
SI  - GENBANK/JN165086
SI  - GENBANK/JN165087
SI  - GENBANK/JN165088
SI  - GENBANK/JN165089
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Acta Virol
JT  - Acta virologica
JID - 0370401
SB  - IM
MH  - Base Sequence
MH  - Begomovirus/classification/*genetics/*isolation & purification
MH  - *Genome, Viral
MH  - Ghana
MH  - Manihot/*virology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Plant Diseases/*virology
MH  - Sequence Analysis, DNA
EDAT- 2012/12/15 06:00
MHDA- 2013/03/21 06:00
CRDT- 2012/12/15 06:00
PHST- 2012/12/15 06:00 [entrez]
PHST- 2012/12/15 06:00 [pubmed]
PHST- 2013/03/21 06:00 [medline]
AID - 10.4149/av_2012_04_305 [doi]
PST - ppublish
SO  - Acta Virol. 2012;56(4):305-14. doi: 10.4149/av_2012_04_305.