PMID- 23238245
OWN - NLM
STAT- MEDLINE
DCOM- 20130624
LR  - 20181202
IS  - 1877-9603 (Electronic)
IS  - 1877-959X (Linking)
VI  - 4
IP  - 1-2
DP  - 2013 Feb
TI  - Localization of the bacterial symbiont Candidatus Midichloria mitochondrii within 
      the hard tick Ixodes ricinus by whole-mount FISH staining.
PG  - 39-45
LID - S1877-959X(12)00060-X [pii]
LID - 10.1016/j.ttbdis.2012.06.005 [doi]
AB  - Here, we present an investigation on the spatial distribution of the bacterial 
      symbiont Candidatus Midichloria mitochondrii within Ixodes ricinus, by whole 
      mount fluorescence in situ hybridization (FISH). M. mitochondrii is a peculiar, 
      recently discovered bacterium that resides in the mitochondria of female ticks. 
      We applied a rapid and specific FISH protocol with oligonucleotide probes 
      targeted on the 16S rRNA of M. mitochondrii, 12S rRNA of tick mitochondria, and a 
      probe revealing active mitochondria. In this report that represents the first 
      application of whole mount FISH on ticks, we observed strong, specific 
      fluorescence signals in all the examined life stages, as the optimized protocol 
      allowed us to overcome the autofluorescence interference of the cuticle. Cellular 
      localization and quantification of the symbionts were also assessed with electron 
      microscopy and specific real-time PCR, respectively.
CI  - Copyright (c) 2012 Elsevier GmbH. All rights reserved.
FAU - Epis, Sara
AU  - Epis S
AD  - Dipartimento di Scienze Veterinarie e Sanita Pubblica, Sezione di Parassitologia, 
      Universita degli Studi di Milano, Via Celoria 10, Milan, Italy.
FAU - Mandrioli, Mauro
AU  - Mandrioli M
FAU - Genchi, Marco
AU  - Genchi M
FAU - Montagna, Matteo
AU  - Montagna M
FAU - Sacchi, Luciano
AU  - Sacchi L
FAU - Pistone, Dario
AU  - Pistone D
FAU - Sassera, Davide
AU  - Sassera D
LA  - eng
PT  - Journal Article
DEP - 20121210
PL  - Netherlands
TA  - Ticks Tick Borne Dis
JT  - Ticks and tick-borne diseases
JID - 101522599
RN  - 0 (RNA, Mitochondrial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Rhodopsins, Microbial)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Alphaproteobacteria/*classification/*isolation & purification
MH  - Animals
MH  - Female
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Ixodes/*microbiology/ultrastructure
MH  - RNA/genetics
MH  - RNA, Mitochondrial
MH  - RNA, Ribosomal, 16S/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Rhodopsins, Microbial
MH  - Symbiosis
EDAT- 2012/12/15 06:00
MHDA- 2013/06/26 06:00
CRDT- 2012/12/15 06:00
PHST- 2012/01/09 00:00 [received]
PHST- 2012/05/24 00:00 [revised]
PHST- 2012/06/05 00:00 [accepted]
PHST- 2012/12/15 06:00 [entrez]
PHST- 2012/12/15 06:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
AID - S1877-959X(12)00060-X [pii]
AID - 10.1016/j.ttbdis.2012.06.005 [doi]
PST - ppublish
SO  - Ticks Tick Borne Dis. 2013 Feb;4(1-2):39-45. doi: 10.1016/j.ttbdis.2012.06.005. 
      Epub 2012 Dec 10.