PMID- 23239594
OWN - NLM
STAT- MEDLINE
DCOM- 20130930
LR  - 20240318
IS  - 1613-6829 (Electronic)
IS  - 1613-6810 (Print)
IS  - 1613-6810 (Linking)
VI  - 9
IP  - 7
DP  - 2013 Apr 8
TI  - Single quantum dot analysis enables multiplexed point mutation detection by gap 
      ligase chain reaction.
PG  - 1096-105
LID - 10.1002/smll.201202242 [doi]
AB  - Gene point mutations present important biomarkers for genetic diseases. However, 
      existing point mutation detection methods suffer from low sensitivity, 
      specificity, and a tedious assay processes. In this report, an assay technology 
      is proposed which combines the outstanding specificity of gap ligase chain 
      reaction (Gap-LCR), the high sensitivity of single-molecule coincidence 
      detection, and the superior optical properties of quantum dots (QDs) for 
      multiplexed detection of point mutations in genomic DNA. Mutant-specific ligation 
      products are generated by Gap-LCR and subsequently captured by QDs to form DNA-QD 
      nanocomplexes that are detected by single-molecule spectroscopy (SMS) through 
      multi-color fluorescence burst coincidence analysis, allowing for multiplexed 
      mutation detection in a separation-free format. The proposed assay is capable of 
      detecting zeptomoles of KRAS codon 12 mutation variants with near 100% 
      specificity. Its high sensitivity allows direct detection of KRAS mutation in 
      crude genomic DNA without PCR pre-amplification.
CI  - Copyright (c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Song, Yunke
AU  - Song Y
AD  - Department of Biomedical Engineering, The Johns Hopkins University School of 
      Medicine, 3400 N. Charles Street, Baltimore, MD 21218, USA.
FAU - Zhang, Yi
AU  - Zhang Y
FAU - Wang, Tza-Huei
AU  - Wang TH
LA  - eng
GR  - R01 CA155305/CA/NCI NIH HHS/United States
GR  - U54 CA151838/CA/NCI NIH HHS/United States
GR  - R01CA155305/CA/NCI NIH HHS/United States
GR  - U54CA151838/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121213
PL  - Germany
TA  - Small
JT  - Small (Weinheim an der Bergstrasse, Germany)
JID - 101235338
SB  - IM
MH  - Biosensing Techniques
MH  - Humans
MH  - Ligase Chain Reaction/*methods
MH  - Point Mutation/*genetics
MH  - *Quantum Dots
PMC - PMC3963288
MID - NIHMS511028
EDAT- 2012/12/15 06:00
MHDA- 2013/10/01 06:00
PMCR- 2014/04/08
CRDT- 2012/12/15 06:00
PHST- 2012/09/12 00:00 [received]
PHST- 2012/12/15 06:00 [entrez]
PHST- 2012/12/15 06:00 [pubmed]
PHST- 2013/10/01 06:00 [medline]
PHST- 2014/04/08 00:00 [pmc-release]
AID - 10.1002/smll.201202242 [doi]
PST - ppublish
SO  - Small. 2013 Apr 8;9(7):1096-105. doi: 10.1002/smll.201202242. Epub 2012 Dec 13.