PMID- 23240064
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 12
DP  - 2012
TI  - Biodegradable nanoparticle-entrapped vaccine induces cross-protective immune 
      response against a virulent heterologous respiratory viral infection in pigs.
PG  - e51794
LID - 10.1371/journal.pone.0051794 [doi]
LID - e51794
AB  - Biodegradable nanoparticle-based vaccine development research is unexplored in 
      large animals and humans. In this study, we illustrated the efficacy of 
      nanoparticle-entrapped UV-killed virus vaccine against an economically important 
      respiratory viral disease of pigs called porcine reproductive and respiratory 
      syndrome virus (PRRSV). We entrapped PLGA [poly (lactide-co-glycolides)] 
      nanoparticles with killed PRRSV antigens (Nano-KAg) and detected its phagocytosis 
      by pig alveolar macrophages. Single doses of Nano-KAg vaccine administered 
      intranasally to pigs upregulated innate and PRRSV specific adaptive responses. In 
      a virulent heterologous PRRSV challenge study, Nano-KAg vaccine significantly 
      reduced the lung pathology and viremia, and the viral load in the lungs. 
      Immunologically, enhanced innate and adaptive immune cell population and 
      associated cytokines with decreased secretion of immunosuppressive mediators were 
      observed at both mucosal sites and blood. In summary, we demonstrated the 
      benefits of intranasal delivery of nanoparticle-based viral vaccine in eliciting 
      cross-protective immune response in pigs, a potential large animal model.
FAU - Dwivedi, Varun
AU  - Dwivedi V
AD  - Food Animal Health Research Program, Ohio Agricultural Research and Development 
      Center, The Ohio State University, Wooster, Ohio, United States.
FAU - Manickam, Cordelia
AU  - Manickam C
FAU - Binjawadagi, Basavaraj
AU  - Binjawadagi B
FAU - Joyappa, Dechamma
AU  - Joyappa D
FAU - Renukaradhya, Gourapura J
AU  - Renukaradhya GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121211
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Administration, Intranasal
MH  - Animals
MH  - Drug Delivery Systems
MH  - Lung/immunology/virology
MH  - *Nanoparticles/administration & dosage/chemistry
MH  - *Porcine Reproductive and Respiratory Syndrome/drug therapy/immunology/prevention 
      & control
MH  - *Porcine respiratory and reproductive syndrome virus/immunology/pathogenicity
MH  - *Respiratory Tract Infections/drug therapy/immunology/prevention & 
      control/veterinary/virology
MH  - Sus scrofa/immunology
MH  - Swine
MH  - *Viral Vaccines/administration & dosage/chemistry/immunology
PMC - PMC3519908
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/12/15 06:00
MHDA- 2013/06/01 06:00
PMCR- 2012/12/11
CRDT- 2012/12/15 06:00
PHST- 2012/08/15 00:00 [received]
PHST- 2012/11/09 00:00 [accepted]
PHST- 2012/12/15 06:00 [entrez]
PHST- 2012/12/15 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
PHST- 2012/12/11 00:00 [pmc-release]
AID - PONE-D-12-24636 [pii]
AID - 10.1371/journal.pone.0051794 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(12):e51794. doi: 10.1371/journal.pone.0051794. Epub 2012 Dec 11.