PMID- 23240628
OWN - NLM
STAT- MEDLINE
DCOM- 20130926
LR  - 20220408
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 81
IP  - 2
DP  - 2013 Feb
TI  - Loop-mediated isothermal amplification for detection of HLA-B*58:01 allele.
PG  - 83-92
LID - 10.1111/tan.12042 [doi]
AB  - Strong association of human leukocyte antigen (HLA)-B*58:01 allele with 
      allopurinol-induced hypersensitivity was found worldwide, especially in the Han 
      Chinese populations. This study aims to develop and evaluate a loop-mediated 
      isothermal amplification (LAMP) assay for rapid detection of HLA-B*58:01. Two 
      sets of LAMP primers targeting exons 2 and 3 of HLA-B*58:01 allele were designed 
      and their annealing temperatures were optimized accordingly. The heating devices 
      for LAMP assay were tested. The analytical sensitivities of the two sets of LAMP 
      primers were determined by 1:10 serial dilution of a positive control with 
      homozygous HLA-B*58:01 allele from 100 ng down to 1 fg. The analytical 
      specificities of the LAMP primers were evaluated by 30 selected University of 
      California, Los Angeles (UCLA) DNA Exchange Program samples with known HLA-B loci 
      typings previously typed by sequencing. Both sets of LAMP primers targeting exons 
      2 and 3 amplified optimally at 67 degrees C. Thermal cycler is essential in achieving a 
      more precise and specific LAMP result. The sensitivity of the exon 2 LAMP primer 
      set was found to be 1 pg, whereas it was 10 ng for the exon 3 primer set in a 
      60-min amplification. The LAMP primers were highly specific because LAMP results 
      were perfectly concordant to the sequencing results. The HLA-B*58:01 LAMP assay 
      has compatible sensitivity and specificity to routine genotyping assays, and it 
      is potentially an alternative screening test for the detection of HLA-B*58:01 and 
      ultimately allopurinol-induced hypersensitivity.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Kwok, J
AU  - Kwok J
AD  - Division of Transplantation and Immunogenetics, Department of Pathology and 
      Clinical Biochemistry, Queen Mary Hospital, Hong Kong SAR, China. 
      kwoksy@ha.org.hk
FAU - Kwong, K M
AU  - Kwong KM
LA  - eng
PT  - Journal Article
DEP - 20121213
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (DNA Primers)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B*58:01 antigen)
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - DNA Primers/metabolism
MH  - Exons/genetics
MH  - HLA-B Antigens/*analysis/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nucleic Acid Amplification Techniques/methods
MH  - Temperature
EDAT- 2012/12/18 06:00
MHDA- 2013/09/27 06:00
CRDT- 2012/12/18 06:00
PHST- 2012/03/01 00:00 [received]
PHST- 2012/11/08 00:00 [revised]
PHST- 2012/11/11 00:00 [accepted]
PHST- 2012/12/18 06:00 [entrez]
PHST- 2012/12/18 06:00 [pubmed]
PHST- 2013/09/27 06:00 [medline]
AID - 10.1111/tan.12042 [doi]
PST - ppublish
SO  - Tissue Antigens. 2013 Feb;81(2):83-92. doi: 10.1111/tan.12042. Epub 2012 Dec 13.