PMID- 2324264
OWN - NLM
STAT- MEDLINE
DCOM- 19900515
LR  - 20190510
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 5
IP  - 2
DP  - 1990 Feb
TI  - Propanediol alters intracellular pH and developmental potential of mouse zygotes 
      independently of volume change.
PG  - 212-6
AB  - Prior to treatment, mouse zygotes were incubated with acridine orange (AO) a 
      fluorescent dye which fluoresces within a physiological pH range. The zygotes 
      were then perifused with 1,2-propanediol (PROH) at rates of 0.18 mol/min, 0.36 
      mol/min and 1.0 mol/min and zygote volume and intracellular pH monitored. Zygotes 
      perifused with phosphate-buffered saline maintained their initial volume and AO 
      fluorescence. All of the zygotes exposed to PROH at a rate of 1 mol/min decreased 
      in volume and lost their AO fluorescence by 10 min. The volume of the zygotes 
      perifused at less than or equal to 0.36 mol/min was not altered. However, only 
      25% of the zygotes perifused at 0.36 mol/min maintained their fluorescence at 10 
      min and all lost their fluorescence by 15 min. At 0.18 mol/min 95% of the zygotes 
      maintained their fluorescence at 10 min and 49% at 15 min. All of the 
      PROH-exposed zygotes lost their fluorescence by 20 min. Although 2-cell 
      development was not affected by 3.0 M PROH for 2.5 min, blastocyst development 
      was reduced compared with controls (P less than 0.05). Longer exposures resulted 
      in a significant decrease in both 2-cell and blastocyst development. These data 
      demonstrate that a 2-7-min exposure to greater than or equal to 2.5 M PROH alters 
      both the intracellular pH and developmental potential. Since these detrimental 
      effects are independent of volume changes and therefore intracellular PROH 
      concentrations, it is postulated that PROH mediates its toxic action by directly 
      altering the cell membrane.
FAU - Damien, M
AU  - Damien M
AD  - Department of Obstetrics and Gynecology, University of Connecticut Health Center, 
      Farmington 06032.
FAU - Luciano, A A
AU  - Luciano AA
FAU - Peluso, J J
AU  - Peluso JJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Propylene Glycols)
RN  - 6DC9Q167V3 (Propylene Glycol)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Embryonic and Fetal Development
MH  - Female
MH  - Hydrogen/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Intracellular Membranes/*metabolism
MH  - Mice
MH  - Propylene Glycol
MH  - Propylene Glycols/*pharmacology
MH  - Time Factors
MH  - Zygote/cytology/drug effects/*physiology
EDAT- 1990/02/01 00:00
MHDA- 1990/02/01 00:01
CRDT- 1990/02/01 00:00
PHST- 1990/02/01 00:00 [pubmed]
PHST- 1990/02/01 00:01 [medline]
PHST- 1990/02/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a137072 [doi]
PST - ppublish
SO  - Hum Reprod. 1990 Feb;5(2):212-6. doi: 10.1093/oxfordjournals.humrep.a137072.