PMID- 23243882
OWN - NLM
STAT- MEDLINE
DCOM- 20140224
LR  - 20161018
IS  - 0250-3301 (Print)
IS  - 0250-3301 (Linking)
VI  - 33
IP  - 9
DP  - 2012 Sep
TI  - [Enrichment and identification of anaerobic ammonium oxidation bacteria].
PG  - 3208-13
AB  - Anaerobic ammonium oxidation (ANAMMOX) bacteria were investigated by cultivation 
      and molecular approaches. Primary enrichments were made with NH4HCO3 and NaNO2 as 
      substrates and two types of sludge from ANAMMOX reactors as inoculum. Two stable 
      enrichment cultures were obtained after two years of successive transfers, and 
      ammonium removal rate was 85%. The ANAMMOX bacteria in the enrichment cultures 
      were identified by Planctomycetales-specific 16S rRNA gene-cloning analysis and 
      fluorescence in situ hybridization (FISH). The clone analysis revealed that all 
      the clonal sequences obtained from the two enrichment cultures were similar and 
      closely related to Candidatus "Kuenenia stuttgartiensis" (99% similarities in 16S 
      rRNA gene sequence). FISH with Planctomycetales-specific probe Pla46 and K. 
      stuttgartiensis-specific probe Kst1275 revealed that the predominant ANAMMOX 
      bacteria in the two enrichment cultures showed positive signals with the Pla46 
      and Kst1275 probes, respectively. To detect the variation in abundance of K. 
      stuttgartiensis in the original inoculum reactor during two years of operation, 
      FISH with Pla46 and Kst1275 probes was applied to the same sludge. The results 
      showed that the abundance of K. stuttgartiensis in the sludge was increased from 
      11% to 24% within two years.
FAU - Liu, Dong-Ying
AU  - Liu DY
AD  - Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess 
      Technology, Chinese Academy of Sciences, Qingdao 266101, China. 
      liudy@qibebt.ac.cn
FAU - Qiu, Yan-Ling
AU  - Qiu YL
FAU - Yuan, Xian-Zheng
AU  - Yuan XZ
FAU - Shi, Xiao-Shuang
AU  - Shi XS
FAU - Guo, Rong-Bo
AU  - Guo RB
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Huan Jing Ke Xue
JT  - Huan jing ke xue= Huanjing kexue
JID - 8405344
RN  - 0 (Nitrites)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Sewage)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Bacteria, Anaerobic/genetics/growth & development/*isolation & 
      purification/metabolism
MH  - Bioreactors
MH  - Nitrites/*metabolism
MH  - Nitrogen/*metabolism
MH  - Oxidation-Reduction
MH  - Quaternary Ammonium Compounds/*metabolism
MH  - Sewage/chemistry/*microbiology
EDAT- 2012/12/19 06:00
MHDA- 2014/02/25 06:00
CRDT- 2012/12/19 06:00
PHST- 2012/12/19 06:00 [entrez]
PHST- 2012/12/19 06:00 [pubmed]
PHST- 2014/02/25 06:00 [medline]
PST - ppublish
SO  - Huan Jing Ke Xue. 2012 Sep;33(9):3208-13.