PMID- 23244775
OWN - NLM
STAT- MEDLINE
DCOM- 20130806
LR  - 20200930
IS  - 1618-1328 (Electronic)
IS  - 0176-1617 (Linking)
VI  - 170
IP  - 4
DP  - 2013 Mar 1
TI  - Sucrose transport is inhibited by okadaic acid during regeneration of 
      sugar-starved Vicia faba root meristem cells.
PG  - 397-405
LID - S0176-1617(12)00509-3 [pii]
LID - 10.1016/j.jplph.2012.10.013 [doi]
AB  - The sucrose-induced resumption of cell cycle in the Vicia faba root meristem 
      cells, blocked in two principal control points PCP1/2 by carbohydrate starvation, 
      occurs after 12 h of metabolic regeneration comprising increased activity of 
      sucrose synthase (SuSy) and hexokinase (HK) as well as starch grain and cell wall 
      matrix polysaccharide biosynthesis. Okadaic acid (OA), the specific protein 
      phosphatase 1/2A inhibitor, supplied at the beginning of the recovery period (0-3 
      h) completely blocks these processes, making cell cycle resumption impossible. On 
      the other hand, when added at the end (9-12 h), OA has a weak inhibitory effect. 
      The aim of these studies was: (1) to establish how sucrose is transported into 
      the cells and whether the above-mentioned effects are correlated with the 
      intensity of its uptake at the beginning and at the end of the metabolic 
      regeneration; and (2) to determine whether OA, blocking sucrose metabolism, also 
      interferes with the process of sucrose uptake and distribution. The level of 
      [(3)H]sucrose uptake was measured by liquid scintillation counting while sugar 
      distribution was analyzed using microautoradiography and electron microscopy. The 
      results showed that sucrose entered the meristematic cells along symplastic or 
      apoplastic pathways and, to a lesser extent, through endocytosis. The cytoplasmic 
      compartments (endoplasmic reticulum, vacuoles, plastids) and the nucleus were 
      labeled. The intensity of [(3)H]sucrose uptake was nearly 2-fold lower during the 
      initial than during the final period of metabolic regeneration. OA inhibited the 
      apoplastic pathway of radioactive molecule uptake and its distribution between 
      cell compartments, implicating PP1/2A involvement in the regulation of this 
      transport.
CI  - Copyright (c) 2012 Elsevier GmbH. All rights reserved.
FAU - Polit, Justyna T
AU  - Polit JT
AD  - Department of Cytophysiology, University of Lodz, ul. Pomorska 141/143, 90-236 
      Lodz, Poland. politj@biol.uni.lodz.pl
FAU - Nazarski, Ryszard B
AU  - Nazarski RB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121212
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - 0 (Carbohydrates)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Plant Growth Regulators)
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - 57-50-1 (Sucrose)
RN  - EC 2.4.1.- (Glucosyltransferases)
RN  - EC 2.4.1.13 (sucrose synthase)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Biological Transport
MH  - Carbohydrates/deficiency
MH  - Cell Cycle
MH  - Enzyme Inhibitors/*metabolism
MH  - Glucosyltransferases/metabolism
MH  - Hexokinase/metabolism
MH  - Okadaic Acid/*metabolism
MH  - Plant Growth Regulators/metabolism
MH  - Plant Root Cap/*growth & development/*metabolism
MH  - Sucrose/*pharmacokinetics
MH  - Vicia faba/*growth & development/*metabolism
EDAT- 2012/12/19 06:00
MHDA- 2013/08/07 06:00
CRDT- 2012/12/19 06:00
PHST- 2012/07/18 00:00 [received]
PHST- 2012/10/03 00:00 [revised]
PHST- 2012/10/04 00:00 [accepted]
PHST- 2012/12/19 06:00 [entrez]
PHST- 2012/12/19 06:00 [pubmed]
PHST- 2013/08/07 06:00 [medline]
AID - S0176-1617(12)00509-3 [pii]
AID - 10.1016/j.jplph.2012.10.013 [doi]
PST - ppublish
SO  - J Plant Physiol. 2013 Mar 1;170(4):397-405. doi: 10.1016/j.jplph.2012.10.013. 
      Epub 2012 Dec 12.