PMID- 23246480
OWN - NLM
STAT- MEDLINE
DCOM- 20131025
LR  - 20131121
IS  - 1879-3185 (Electronic)
IS  - 0300-483X (Linking)
VI  - 311
IP  - 1-2
DP  - 2013 Sep 6
TI  - Sex-dependent effects of early maternal deprivation on MDMA-induced conditioned 
      place preference in adolescent rats: possible neurochemical correlates.
PG  - 78-86
LID - S0300-483X(12)00422-2 [pii]
LID - 10.1016/j.tox.2012.12.003 [doi]
AB  - The early neonatal stage constitutes a sensitive period during which exposure to 
      adverse events can increase the risk of neuropsychiatric disorders. Maternal 
      deprivation (MD) is a model of early life stress that induces long-term 
      behavioural and physiological alterations, including susceptibility to different 
      drugs of abuse. In the present study we have used the conditioned place 
      preference (CPP) paradigm to address the influence of MD on the rewarding effects 
      of 3,4-methylenedioxymetamphetamine (MDMA) in adolescent animals of both sexes. 
      We have previously observed in adolescent rats that MD induces modifications in 
      the serotonergic and endocannabinoid systems, which play a role in the rewarding 
      effects of MDMA. In light of this evidence, we hypothesized that MD would alter 
      the psychobiological consequences of exposure to MDMA. Neonatal Wistar rats 
      underwent MD (24h, on PND 9) or were left undisturbed (controls). The animals 
      were conditioned with 2.5mg/kg MDMA during the periadolescent period (PND 34-PND 
      43) and were tested in the open-field test at the end of adolescence (PND 60). 
      Animals were sacrificed on PND 68-75 and levels of serotonin (5-HT) and its 
      metabolite 5-hydroxyindole acetic acid were measured in the striatum, hippocampus 
      and cortex, while the expression of hippocampal CB1 cannabinoid receptor (CB1R) 
      and circulating levels of corticosterone and leptin were also measured. Control 
      males showed CPP after administration of MDMA. However, no MDMA-induced CPP was 
      detected in control females or MD males, and MD had no effect on open field 
      activity in any group. A reduction in striatal and cortical 5-HT levels, 
      increased expression of hippocampal CB1R and a marked trend towards higher 
      circulating leptin levels were observed in MDMA-treated MD males. Our results 
      demonstrate for the first time that MD reduces the rewarding effects of MDMA in a 
      sex-dependent manner. We propose that this effect is related, at least in part, 
      with alterations of the serotonergic and cannabinoid systems.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Llorente-Berzal, Alvaro
AU  - Llorente-Berzal A
AD  - Depto. Fisiologia (Fisiologia Animal II), Fac Biologia, Universidad Complutense, 
      Madrid, Spain.
FAU - Manzanedo, Carmen
AU  - Manzanedo C
FAU - Daza-Losada, Manuel
AU  - Daza-Losada M
FAU - Valero, Manuel
AU  - Valero M
FAU - Lopez-Gallardo, Meritxell
AU  - Lopez-Gallardo M
FAU - Aguilar, Maria A
AU  - Aguilar MA
FAU - Rodriguez-Arias, Marta
AU  - Rodriguez-Arias M
FAU - Minarro, Jose
AU  - Minarro J
FAU - Viveros, Maria-Paz
AU  - Viveros MP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121214
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Aging/drug effects/physiology
MH  - Animals
MH  - Brain/*drug effects/metabolism
MH  - Brain Chemistry/*drug effects/physiology
MH  - Conditioning, Classical/*drug effects/physiology
MH  - Female
MH  - Male
MH  - *Maternal Deprivation
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*toxicity
MH  - Rats
MH  - Rats, Wistar
MH  - *Sex Characteristics
OTO - NOTNLM
OT  - Adolescence
OT  - CB(1) receptor
OT  - Conditioned place preference
OT  - MDMA
OT  - Maternal deprivation
OT  - Serotonergic system
EDAT- 2012/12/19 06:00
MHDA- 2013/10/26 06:00
CRDT- 2012/12/19 06:00
PHST- 2012/09/25 00:00 [received]
PHST- 2012/12/04 00:00 [revised]
PHST- 2012/12/05 00:00 [accepted]
PHST- 2012/12/19 06:00 [entrez]
PHST- 2012/12/19 06:00 [pubmed]
PHST- 2013/10/26 06:00 [medline]
AID - S0300-483X(12)00422-2 [pii]
AID - 10.1016/j.tox.2012.12.003 [doi]
PST - ppublish
SO  - Toxicology. 2013 Sep 6;311(1-2):78-86. doi: 10.1016/j.tox.2012.12.003. Epub 2012 
      Dec 14.