PMID- 23248968
OWN - NLM
STAT- MEDLINE
DCOM- 20130122
LR  - 20121219
IS  - 0535-5133 (Print)
IS  - 0535-5133 (Linking)
VI  - 53
IP  - 3
DP  - 2012 Sep
TI  - [Effect of Leishmania mexicana, RANTES and TNF alpha on splenic dendritic cells 
      of newborn and adult BALB/c mice: phenotypic characteristics and migratory 
      properties].
PG  - 237-49
AB  - Dendritic cells (DCs) are essential in the development and regulation of the 
      immune response (IR). The inherent potential of DCs to induce a specific immune 
      response in the neonatal period is controversial. It has been suggested that the 
      specific IR in neonates depends on the quantitative relation of DC/T lymphocytes, 
      as well as on the neonatal or adult age at which the interaction antigen/DC/T 
      lymphocytes occurs. This suggests that this contact has an influence on the 
      phenotypic and/or biological properties of DCs, which modifies its behavior. 
      Therefore, the effects of Leishmania mexicana (L. mexicana) and of TNFalpha and 
      RANTES cytokines on immunophenotypical characteristics were evaluated on spleen 
      DCs, from neonate and adult BALB/c mice, by using flow cytometry and in vitro 
      migratory properties with a Boyden Chamber. In basal conditions, neonate and 
      adult DCs express the same molecules (CD40, CD86, MHCII and CD54). When the DCs 
      interact with the antigen L. mexicana, the expression of these molecules are 
      similar in adults and in neonates, with the exception of CD40 whose intensity of 
      expression was raised (P < 0.05) in both groups. The rate of migration of the DCs 
      in a culture medium conditioned of L. mexicana, RANTES and TNFalpha was higher in 
      adults than in newborn mice. These observations suggest that neonatal and adult 
      mice DCs have similar phenotypic characteristics. Under the effect of the same 
      stimulus they respond differently; suggesting that other factors are involved in 
      the higher susceptibility that newborns have to infections.
FAU - Verzura, Julie
AU  - Verzura J
AD  - Unidad de Investigacion en Inmunologia (UNIVENIN), Departamento de Ciencias 
      Fisiologicas, Facultad de Ciencias de la Salud, Universidad de Carabobo, Barbula, 
      Venezuela.
FAU - Ponce, Loida
AU  - Ponce L
FAU - Pernia, Monica
AU  - Pernia M
FAU - Mendoza, Francisco
AU  - Mendoza F
FAU - Tovar, Robert
AU  - Tovar R
FAU - de Orta, Sioly
AU  - de Orta S
FAU - Corado, Jose
AU  - Corado J
LA  - spa
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
TT  - Efecto de la Leishmania mexicana, RANTES y TNF alfa en el fenotipo y propiedades 
      migratorias in vitro de celulas dendriticas esplenicas de ratones BALB/c neonatos 
      y adultos.
PL  - Venezuela
TA  - Invest Clin
JT  - Investigacion clinica
JID - 0421531
RN  - 0 (Chemokine CCL5)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - *Cell Movement
MH  - Chemokine CCL5/*physiology
MH  - Dendritic Cells/*parasitology/*physiology
MH  - Female
MH  - Humans
MH  - Leishmania mexicana/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phenotype
MH  - Spleen/*cytology
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 2012/12/20 06:00
MHDA- 2013/01/23 06:00
CRDT- 2012/12/20 06:00
PHST- 2012/12/20 06:00 [entrez]
PHST- 2012/12/20 06:00 [pubmed]
PHST- 2013/01/23 06:00 [medline]
PST - ppublish
SO  - Invest Clin. 2012 Sep;53(3):237-49.