PMID- 23250574
OWN - NLM
STAT- MEDLINE
DCOM- 20130807
LR  - 20130227
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Linking)
VI  - 351
IP  - 3
DP  - 2013 Mar
TI  - Peripheral choline acetyltransferase in rat skin demonstrated by 
      immunohistochemistry.
PG  - 497-510
LID - 10.1007/s00441-012-1536-z [doi]
AB  - Conventional choline acetyltransferase immunohistochemistry has been used widely 
      for visualizing central cholinergic neurons and fibers but not often for labeling 
      peripheral structures, probably because of their poor staining. The recent 
      identification of the peripheral type of choline acetyltransferase (pChAT) has 
      enabled the clear immunohistochemical detection of many known peripheral 
      cholinergic elements. Here, we report the presence of pChAT-immunoreactive nerve 
      fibers in rat skin. Intensely stained nerve fibers were distributed in 
      association with eccrine sweat glands, blood vessels, hair follicles and portions 
      just beneath the epidermis. These results suggest that pChAT-positive nerves 
      participate in the sympathetic cholinergic innervation of eccrine sweat glands. 
      Moreover, pChAT also appears to play a role in cutaneous sensory nerve endings. 
      These findings are supported by the presence of many pChAT-positive neuronal 
      cells in the sympathetic ganglion and dorsal root ganglion. Thus, pChAT 
      immunohistochemistry should provide a novel and unique tool for studying 
      cholinergic nerves in the skin.
FAU - Hanada, Keiji
AU  - Hanada K
AD  - Department of Dermatology, Kyoto Prefectural University of Medicine, 465 
      Kajii-cho, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto, 602-8566, Japan. 
      hanada-k@koto.kpu-m.ac.jp
FAU - Kishimoto, Saburo
AU  - Kishimoto S
FAU - Bellier, Jean-Pierre
AU  - Bellier JP
FAU - Kimura, Hiroshi
AU  - Kimura H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121219
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Neuronal Tract-Tracers)
RN  - 0 (Vesicular Acetylcholine Transport Proteins)
RN  - 33507-63-0 (Substance P)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Animals
MH  - Choline O-Acetyltransferase/*metabolism
MH  - Eccrine Glands/enzymology/innervation
MH  - Ganglia, Spinal/cytology/enzymology/surgery
MH  - Ganglia, Sympathetic/cytology/enzymology/surgery
MH  - Ganglionectomy
MH  - Immunohistochemistry
MH  - Nerve Fibers/metabolism
MH  - Neuronal Tract-Tracers
MH  - Peripheral Nerves/cytology/*enzymology
MH  - Rats
MH  - Rats, Wistar
MH  - Skin/*enzymology/*innervation
MH  - Substance P/metabolism
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - Vesicular Acetylcholine Transport Proteins/metabolism
EDAT- 2012/12/20 06:00
MHDA- 2013/08/08 06:00
CRDT- 2012/12/20 06:00
PHST- 2012/07/17 00:00 [received]
PHST- 2012/11/22 00:00 [accepted]
PHST- 2012/12/20 06:00 [entrez]
PHST- 2012/12/20 06:00 [pubmed]
PHST- 2013/08/08 06:00 [medline]
AID - 10.1007/s00441-012-1536-z [doi]
PST - ppublish
SO  - Cell Tissue Res. 2013 Mar;351(3):497-510. doi: 10.1007/s00441-012-1536-z. Epub 
      2012 Dec 19.