PMID- 23251235
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240109
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 5
IP  - 1
DP  - 2013 Jan
TI  - Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon 
      tumor growth in mice.
PG  - 17-22
AB  - Previously, we observed that purified monogalactosyl diacylglycerol (MGDG), a 
      major glycoglycerolipid from spinach, selectively inhibits the activities of 
      mammalian replicative DNA polymerases (alpha, delta and epsilon). However, the function of MGDG 
      following ingestion is not well-known. In the present study, spinach MGDG 
      suppressed the proliferation of Colon26 mouse colon cancer cells with an LD(50) 
      of 24 mug/ml in vitro. gamma-cyclodextrin (CD)-MGDG complex was prepared and 
      administered orally following Colon26 mouse tumor adhesion for 26 days. It was 
      observed that 20 mg/kg equivalent (eq.) of the CD-MGDG complex reduced tumor 
      volume by  approximately 60% compared with that of the vehicle-treated controls. In 
      immunohistochemical analysis, the CD-MGDG complex group showed a decreased number 
      of proliferating cell nuclear antigen (PCNA)-positive cells and reduction of 
      mitosis in the tumor cells compared with the control group. In addition, the 
      CD-MGDG complex increased the number of terminal deoxynucleotidyl transferase 
      dUTP nick-end labeling (TUNEL)-positive apoptotic cells and inhibited 
      CD31-positive tumor blood vessel growth significantly. These results suggest that 
      MGDG has the potential for cancer prevention and health promotion.
FAU - Maeda, Naoki
AU  - Maeda N
AD  - Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, 
      Kobe-Gakuin University, Kobe, Hyogo 651-2180;
FAU - Kokai, Yasuo
AU  - Kokai Y
FAU - Hada, Takahiko
AU  - Hada T
FAU - Yoshida, Hiromi
AU  - Yoshida H
FAU - Mizushina, Yoshiyuki
AU  - Mizushina Y
LA  - eng
PT  - Journal Article
DEP - 20121101
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC3524182
EDAT- 2012/12/20 06:00
MHDA- 2012/12/20 06:01
PMCR- 2012/11/01
CRDT- 2012/12/20 06:00
PHST- 2012/06/02 00:00 [received]
PHST- 2012/10/02 00:00 [accepted]
PHST- 2012/12/20 06:00 [entrez]
PHST- 2012/12/20 06:00 [pubmed]
PHST- 2012/12/20 06:01 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - etm-05-01-0017 [pii]
AID - 10.3892/etm.2012.792 [doi]
PST - ppublish
SO  - Exp Ther Med. 2013 Jan;5(1):17-22. doi: 10.3892/etm.2012.792. Epub 2012 Nov 1.