PMID- 23252792
OWN - NLM
STAT- MEDLINE
DCOM- 20130927
LR  - 20191210
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 38
IP  - 3
DP  - 2013 Mar
TI  - Hydrogen-rich saline prevents early neurovascular dysfunction resulting from 
      inhibition of oxidative stress in STZ-diabetic rats.
PG  - 396-404
LID - 10.3109/02713683.2012.748919 [doi]
AB  - PURPOSE: Diabetic retinopathy (DR) is characterized by increased oxidative and 
      nitrosative stress, both of which lead to neurotoxicity and vascular 
      permeability. Previous studies on a variety of organs indicate that hydrogen-rich 
      saline not only has considerable antioxidant and anti-inflammatory properties, 
      but also suppresses oxidative stress-induced injury. In the present study, we 
      assessed the effects of hydrogen-rich saline on neurovascular dysfunction and 
      oxidative stress in an animal model (rat) of DR. MATERIALS AND METHODS: Male 
      Sprague-Dawley rats with streptozotocin (STZ)-induced diabetes mellitus (DM) were 
      injected intraperitoneally with 5 ml/kg hydrogen-saturated (experimental) or 
      plain (control) saline daily for one month. Visual function and blood-retinal 
      barrier (BRB) integrity were evaluated by electroretinography (ERG) and bovine 
      serum albumin (BSA)-fluorescence, respectively. Histological changes in the inner 
      retina were assessed by light microscopy. Biomarkers of oxidative stress, 
      including 4-hydroxynonenal (4-HNE) and 8-hydroxy-2-deoxyguanosine (8-OH-dG), and 
      antioxidant enzymes, including superoxide dismutase, glutathione peroxidase, 
      glutathione reductase and glutathione transferase, were evaluated by ELISA. 
      Synaptophysin and brain-derived neurotrophic factor (BDNF) levels were measured 
      by immunoblotting. RESULTS: STZ-diabetic rats were marked by clearly reduced 
      b-wave amplitudes and oscillatory potentials, DM-related BRB breakdown and 
      histological changes in the inner retina, all of which were suppressed following 
      treatment with hydrogen-rich saline. Furthermore, hydrogen-rich saline reduced 
      oxidative stress, increased antioxidant enzyme activities and preserved 
      synaptophysin and BDNF levels in the diabetic rat retina. CONCLUSIONS: Based on 
      its inhibition of oxidative stress and up-regulation of anti-oxidative enzymes, 
      we conclude that hydrogen-rich saline is a potentially valuable therapeutic 
      modality for the treatment of DR.
FAU - Feng, Yanqing
AU  - Feng Y
AD  - Department of Ophthalmology, Shanghai Kongjiang Hospital, Shanghai, China.
FAU - Wang, Ruobing
AU  - Wang R
FAU - Xu, Jiajun
AU  - Xu J
FAU - Sun, Jingchuan
AU  - Sun J
FAU - Xu, Tao
AU  - Xu T
FAU - Gu, Qing
AU  - Gu Q
FAU - Wu, Xingwei
AU  - Wu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121219
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Aldehydes)
RN  - 0 (Blood Glucose)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Synaptophysin)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - EC 1.- (Oxidoreductases)
RN  - G9481N71RO (Deoxyguanosine)
RN  - K1CVM13F96 (4-hydroxy-2-nonenal)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Aldehydes/metabolism
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Blood-Retinal Barrier/drug effects
MH  - Blotting, Western
MH  - Body Weight
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Deoxyguanosine/analogs & derivatives/metabolism
MH  - Diabetes Mellitus, Experimental/metabolism/physiopathology/*prevention & control
MH  - Diabetic Retinopathy/metabolism/physiopathology/*prevention & control
MH  - Electroretinography
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Male
MH  - Oxidative Stress/*drug effects
MH  - Oxidoreductases/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retinal Neovascularization/physiopathology/*prevention & control
MH  - Sodium Chloride/*pharmacology
MH  - Synaptophysin/metabolism
EDAT- 2012/12/21 06:00
MHDA- 2013/09/28 06:00
CRDT- 2012/12/21 06:00
PHST- 2012/12/21 06:00 [entrez]
PHST- 2012/12/21 06:00 [pubmed]
PHST- 2013/09/28 06:00 [medline]
AID - 10.3109/02713683.2012.748919 [doi]
PST - ppublish
SO  - Curr Eye Res. 2013 Mar;38(3):396-404. doi: 10.3109/02713683.2012.748919. Epub 
      2012 Dec 19.