PMID- 23252902
OWN - NLM
STAT- MEDLINE
DCOM- 20130403
LR  - 20220129
IS  - 1749-6632 (Electronic)
IS  - 0077-8923 (Linking)
VI  - 1274
DP  - 2012 Dec
TI  - Antibodies identified by cell-based assays in myasthenia gravis and associated 
      diseases.
PG  - 92-8
LID - 10.1111/j.1749-6632.2012.06789.x [doi]
AB  - We have established cell-based assays for the improved detection of acetylcholine 
      receptor (AChR) and muscle-specific kinase (MuSK) antibodies in myasthenia 
      gravis. This approach has enabled us to demonstrate antibodies to "clustered" 
      AChRs in patients who were previously AChR antibody negative and can also be used 
      to distinguish between adult and fetal AChR antibodies in mothers of babies with 
      arthrogryposis multiplex congenita. We summarize our recent evidence for the 
      pathogenicity of MuSK and clustered AChR antibodies using in vivo models. 
      Cell-based assays are now also being used for the detection of other antibodies, 
      such as those directed to components of the VGKC/CASPR2/LGI1 complex in Morvan's 
      syndrome, and to AQP4 antibodies in neuromyelitis optica; both of these diseases 
      can be associated with MG and sometimes thymoma. The cell-based method is time 
      consuming but has many advantages and may provide a gold standard for the future 
      in the detection of pathogenic autoantibodies in patients with immune-mediated 
      diseases.
CI  - (c) 2012 New York Academy of Sciences.
FAU - Vincent, Angela
AU  - Vincent A
AD  - Nuffield Department of Clinical Neurosciences, University of Oxford, John 
      Radcliffe Hospital, United Kingdom. angela.vincent@ndcn.ox.ac.uk
FAU - Waters, Patrick
AU  - Waters P
FAU - Leite, M Isabel
AU  - Leite MI
FAU - Jacobson, Leslie
AU  - Jacobson L
FAU - Koneczny, Inga
AU  - Koneczny I
FAU - Cossins, Judith
AU  - Cossins J
FAU - Beeson, David
AU  - Beeson D
LA  - eng
GR  - 084655/Wellcome Trust/United Kingdom
GR  - G0701521/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (AQP4 protein, human)
RN  - 0 (Aquaporin 4)
RN  - 0 (Autoantibodies)
RN  - 0 (Receptors, Cholinergic)
RN  - EC 2.7.10.1 (MUSK protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Aquaporin 4/metabolism
MH  - Arthrogryposis/immunology
MH  - Autoantibodies/*immunology
MH  - Biological Assay
MH  - Humans
MH  - Myasthenia Gravis/*immunology/pathology
MH  - Receptor Protein-Tyrosine Kinases/metabolism
MH  - Receptors, Cholinergic/immunology/metabolism
EDAT- 2012/12/21 06:00
MHDA- 2013/04/04 06:00
CRDT- 2012/12/21 06:00
PHST- 2012/12/21 06:00 [entrez]
PHST- 2012/12/21 06:00 [pubmed]
PHST- 2013/04/04 06:00 [medline]
AID - 10.1111/j.1749-6632.2012.06789.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2012 Dec;1274:92-8. doi: 10.1111/j.1749-6632.2012.06789.x.