PMID- 23253727
OWN - NLM
STAT- MEDLINE
DCOM- 20131203
LR  - 20181202
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 125
IP  - 24
DP  - 2012 Dec
TI  - Methods of isolation, expansion, differentiating induction and preservation of 
      human umbilical cord mesenchymal stem cells.
PG  - 4504-10
AB  - OBJECTIVE: This literature review aims to summarize the methods of isolation, 
      expansion, differentiation and preservation of human umbilical cord mesenchymal 
      stem cells (hUCMSCs), for comprehensive understanding and practical use in 
      preclinical research and clinical trials. DATA SOURCES: All the literature 
      reviewed was published over the last 10 years and is listed in PubMed and Chinese 
      National Knowledge Infrastructure (CNKI). Studies were retrieved using the key 
      word "human umbilical cord mesenchymal stem cells". RESULTS: Explants culture and 
      enzymatic digestion are two methods to isolate hUCMSCs from WJ and there are 
      modifications to improve these methods. Culture conditions may affect the 
      expansion and differentiating orientations of hUCMSCs. In addition, hUCMSCs can 
      maintain their multi-potential effects after being properly frozen and thawed. 
      CONCLUSION: Considering their multi-potential, convenient and non-invasive 
      accessibility, low immunogenicity and the reported therapeutic effects in several 
      different preclinical animal models, hUCMSCs have immense scope in regeneration 
      medicine as a substitute for MSCs derived from bone marrow or umbilical cord 
      blood.
FAU - Li, Dong-Rui
AU  - Li DR
AD  - Department of Surgery, Second Hospital of Hebei Medical University, Shijiazhuang, 
      Hebei, China.
FAU - Cai, Jian-Hui
AU  - Cai JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
SB  - IM
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/physiology
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Umbilical Cord/*cytology
EDAT- 2012/12/21 06:00
MHDA- 2013/12/16 06:00
CRDT- 2012/12/21 06:00
PHST- 2012/12/21 06:00 [entrez]
PHST- 2012/12/21 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2012 Dec;125(24):4504-10.