PMID- 23255148
OWN - NLM
STAT- MEDLINE
DCOM- 20140619
LR  - 20240104
IS  - 1574-4647 (Electronic)
IS  - 0161-9152 (Print)
IS  - 0161-9152 (Linking)
VI  - 35
IP  - 6
DP  - 2013 Dec
TI  - BDNF transcripts, proBDNF and proNGF, in the cortex and hippocampus throughout 
      the life span of the rat.
PG  - 2057-70
LID - 10.1007/s11357-012-9495-6 [doi]
AB  - Neurotrophins are established molecular mediators of neuronal plasticity in the 
      adult brain. We analyzed the impact of aging on brain-derived neurotrophic factor 
      (BDNF) and nerve growth factor (NGF) protein isoforms, their receptors, and on 
      the expression patterns of multiple 5' exon-specific BDNF transcripts in the rat 
      cortex and hippocampus throughout the life span of the rat (6, 12, 18, and 24 
      months of age). ProNGF was increased during aging in both structures. Mature NGF 
      gradually decreased in the cortex, and, in 24-month-old animals, it was 30% lower 
      than that in adult 6-month-old rats. The BDNF expression did not change during 
      aging, while proBDNF accumulated in the hippocampus of aged rats. Hippocampal 
      total BDNF mRNA was lower in 12-month-old animals, mostly as a result of a 
      decrease of BDNF transcripts 1 and 2. In contrast to the region-specific 
      regulation of specific exon-containing BDNF mRNAs in adult animals, the same BDNF 
      RNA isoforms (containing exons III, IV, or VI) were present in both brain 
      structures of aged animals. Deficits in neurotrophin signaling were supported by 
      the observed decrease in Trk receptor expression which was accompanied by lower 
      levels of the two main downstream effector kinases, pAkt and protein kinase C. 
      The proteolytic processing of p75NTR observed in 12-month-old rats points to an 
      additional regulatory mechanism in early aging. The changes described herein 
      could contribute to reduced brain plasticity underlying the age-dependent decline 
      in cognitive function.
FAU - Perovic, Milka
AU  - Perovic M
AD  - Institute for Biological Research, University of Belgrade, Bulevar despota 
      Stefana 142, 11060, Belgrade, Serbia.
FAU - Tesic, Vesna
AU  - Tesic V
FAU - Mladenovic Djordjevic, Aleksandra
AU  - Mladenovic Djordjevic A
FAU - Smiljanic, Kosara
AU  - Smiljanic K
FAU - Loncarevic-Vasiljkovic, Natasa
AU  - Loncarevic-Vasiljkovic N
FAU - Ruzdijic, Sabera
AU  - Ruzdijic S
FAU - Kanazir, Selma
AU  - Kanazir S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121221
PL  - Netherlands
TA  - Age (Dordr)
JT  - Age (Dordrecht, Netherlands)
JID - 101250497
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (brain-derived neurotrophic factor precursor)
RN  - 0 (pro-nerve growth factor, rat)
SB  - IM
MH  - Aging/*genetics/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/*genetics
MH  - Cerebral Cortex/*growth & development/metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - Hippocampus/*growth & development/metabolism
MH  - Immunohistochemistry
MH  - Nerve Growth Factors/biosynthesis/*genetics
MH  - Protein Precursors/biosynthesis/*genetics
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Transcription, Genetic
PMC - PMC3824987
EDAT- 2012/12/21 06:00
MHDA- 2014/06/20 06:00
PMCR- 2014/12/01
CRDT- 2012/12/21 06:00
PHST- 2012/06/07 00:00 [received]
PHST- 2012/12/05 00:00 [accepted]
PHST- 2012/12/21 06:00 [entrez]
PHST- 2012/12/21 06:00 [pubmed]
PHST- 2014/06/20 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - 9495 [pii]
AID - 10.1007/s11357-012-9495-6 [doi]
PST - ppublish
SO  - Age (Dordr). 2013 Dec;35(6):2057-70. doi: 10.1007/s11357-012-9495-6. Epub 2012 
      Dec 21.