PMID- 23256388
OWN - NLM
STAT- MEDLINE
DCOM- 20130808
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 8
DP  - 2012 Aug
TI  - [Determination: of citrus red 2 in navel orange by ultra performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 832-5
AB  - An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) 
      method was developed for the determination of citrus red 2 (CR2) in navel orange. 
      The sample was extracted with acetonitrile, cleaned-up by an NH2 solid phase 
      extraction cartridge. Then, the analyte was separated on a Waters Acquity UPLC 
      BEH C18 column (50 mm x 2.1 mm, 1.7 microm) in gradient elution with the mobile 
      phases of water and acetonitrile. The separated compounds were detected with a 
      Waters Xevo TQ MS tandem quadrupole mass spectrometer operated in multiple 
      reaction monitoring (MRM) mode via positive electrospray ionization (ESI+). The 
      linearity of the method was good (r2 = 0.996 5) over the concentration range from 
      0.1 to 100 microg/L for CR2. The limit of detection (LOD) was 0.05 microg/kg, and 
      the limit of quantification (LOQ) was 0.1 microg/kg. The average recoveries of 
      CR2 at the three levels of 1.0, 10.0 and 100.0 microg/kg were between 84.2% and 
      94.0% with the relative standard deviations (RSDs) between 2.86% and 10.15%. 
      Finally, a total of 18 navel orange samples randomly collected from different 
      markets were detected by the proposed method, and two samples contained CR2. The 
      sensitivity, accuracy, and precision of this method can meet the requirements of 
      the CR2 analysis.
FAU - Hu, Li
AU  - Hu L
AD  - Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Chengdu 
      610066, China. huli0704@163.com
FAU - Lei, Shaorong
AU  - Lei S
FAU - Guo, Ling'an
AU  - Guo L
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Azo Compounds)
RN  - 2QE5Y68984 (citrus red No. 2)
SB  - IM
MH  - Azo Compounds/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Citrus sinensis/*chemistry
MH  - Food Contamination/*analysis
MH  - Fruit/chemistry
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/12/22 06:00
MHDA- 2013/08/09 06:00
CRDT- 2012/12/22 06:00
PHST- 2012/12/22 06:00 [entrez]
PHST- 2012/12/22 06:00 [pubmed]
PHST- 2013/08/09 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.03045 [doi]
PST - ppublish
SO  - Se Pu. 2012 Aug;30(8):832-5. doi: 10.3724/sp.j.1123.2012.03045.