PMID- 23256390
OWN - NLM
STAT- MEDLINE
DCOM- 20130808
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 8
DP  - 2012 Aug
TI  - [Simultaneous determination of meso-erythritol and L-erythrulose in fermentation 
      broth using high performance liquid chromatography].
PG  - 843-6
AB  - A high performance liquid chromatographic (HPLC) method has been developed for 
      the simultaneous determination of meso-erythritol and L-erythrulose in 
      fermentation broth. The chromatographic conditions were as follows: Lichrospher 
      5-NH2 column (250 mm x 4.6 mm) with the temperature of 30 degrees C, 
      acetonitrile-water (90: 10, v/v) as mobile phase with the flow rate of 1.0 
      mL/min. meso-Erythritol was detected by refractive index (RI) detector at 35 
      degrees C and L-erythrulose was detected by ultraviolet (UV) detector at 277 nm 
      at room temperature. The linear range for meso-erythritol was 1.00 - 100.00 g/L 
      with a correlation coefficient of 0.998 5. The limit of detection (LOD) and the 
      limit of quantification (LOQ) for meso-erythritol were 0.10 g/L and 0.45 g/L, 
      respectively. The linear range for L-erythrulose was 1.00 - 100.00 g/L with a 
      correlation coefficient of 0.995 8. The LOD and LOQ for L-erythrulose were 0.50 
      g/L and 0.87 g/L, respectively. The relative standard deviations (RSDs) of 
      intraday and interday for meso-erythritol were less than 3.28% and 5.30%, 
      respectively. The intraday and interday RSDs for L-erythrulose were less than 
      2.16% and 2.25%, respectively. The recoveries of meso-erythritol and 
      L-erythrulose in fermentation broth were greater than 99%. The samples from 
      fermentation broth were detected at different time points. The measurement by the 
      novel HPLC method was not affected by the other components in the fermentation 
      broth. Furthermore, the HPLC method can be used for the determination of the 
      substrate meso-erythritol and the product L-erythrulose simultaneously.
FAU - Ge, Chiyu
AU  - Ge C
AD  - School of Life Science and Technology, China Pharmaceutical University, Nanjing 
      210009, China.
FAU - Zhang, Junli
AU  - Zhang J
FAU - Chen, Jianhua
AU  - Chen J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Tetroses)
RN  - 40031-31-0 (erythrulose)
RN  - RA96B954X6 (Erythritol)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Erythritol/*analysis
MH  - *Fermentation
MH  - Tetroses/*analysis/biosynthesis
EDAT- 2012/12/22 06:00
MHDA- 2013/08/09 06:00
CRDT- 2012/12/22 06:00
PHST- 2012/12/22 06:00 [entrez]
PHST- 2012/12/22 06:00 [pubmed]
PHST- 2013/08/09 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.03051 [doi]
PST - ppublish
SO  - Se Pu. 2012 Aug;30(8):843-6. doi: 10.3724/sp.j.1123.2012.03051.