PMID- 23256627
OWN - NLM
STAT- MEDLINE
DCOM- 20140630
LR  - 20191210
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 61
IP  - 10
DP  - 2013 Mar 13
TI  - Rapid method for the determination of multiple mycotoxins in wines and beers by 
      LC-MS/MS using a stable isotope dilution assay.
PG  - 2378-84
LID - 10.1021/jf304729f [doi]
AB  - A "dilute and shoot" method for the liquid chromatography-tandem mass 
      spectrometric (LC-MS/MS) determination of multiple mycotoxins (aflatoxins B1, B2, 
      G1, G2, ochratoxin A (OTA), fumonisins (F) B1 and B2, zearalenone, 
      deoxynivalenol, T-2 toxin, and HT-2 toxin) in wines and beers has been developed 
      and validated. Separation was accomplished using ultrahigh-performance liquid 
      chromatography (UHPLC) with <10 min analysis time. Mycotoxins were detected by 
      dynamic multiple reaction monitoring (MRM) in positive electrospray ionization 
      mode. Due to matrix effects, (13)C-uniformly labeled mycotoxins were added to the 
      sample extracts prior to LC-MS/MS analysis. With external calibration, recoveries 
      were 18-148% for white wines, 15-118% for red wines, and 20-125% for beers, at 
      three spiking levels. The (13)C-labeled internal standards compensated for matrix 
      effects effectively, with overall recoveries of 94-112% for white wines, 80-137% 
      for red wines, and 61-131% for beers, with greater recoveries for FB1 and FB2, at 
      three spiking levels. The relative standard deviation was <20% for all analytes 
      in the wines and beers. This method was applied to a USDA-funded nationwide 
      survey of domestic and imported wines and beers for the determination of OTA and 
      extended to include other mycotoxins.
FAU - Al-Taher, Fadwa
AU  - Al-Taher F
AD  - Institute for Food Safety and Health, Illinois Institute of Technology , 6502 
      South Archer Road, Bedford Park, Illinois 60501, United States.
FAU - Banaszewski, Katie
AU  - Banaszewski K
FAU - Jackson, Lauren
AU  - Jackson L
FAU - Zweigenbaum, Jerry
AU  - Zweigenbaum J
FAU - Ryu, Dojin
AU  - Ryu D
FAU - Cappozzo, Jack
AU  - Cappozzo J
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130122
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Mycotoxins)
SB  - IM
MH  - Beer/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - *Indicator Dilution Techniques
MH  - Mycotoxins/*analysis
MH  - Tandem Mass Spectrometry/*methods
MH  - Wine/*analysis
EDAT- 2012/12/22 06:00
MHDA- 2014/07/01 06:00
CRDT- 2012/12/22 06:00
PHST- 2012/12/22 06:00 [entrez]
PHST- 2012/12/22 06:00 [pubmed]
PHST- 2014/07/01 06:00 [medline]
AID - 10.1021/jf304729f [doi]
PST - ppublish
SO  - J Agric Food Chem. 2013 Mar 13;61(10):2378-84. doi: 10.1021/jf304729f. Epub 2013 
      Jan 22.